Abstract
Creating physical-biochemical superposed microenvironments optimal for stimulating neurite outgrowth would be beneficial for neuronal regenerative medicine. We investigated potential co-regulatory effects of cell micropatterning and retinoic acid (RA) soluble factor on neuronal cell morphology and neurite outgrowth. Human neuroblastoma (SH-SY5Y) cell patterning sensitivity could be enhanced by poly-l-lysine-g-polyethylene glycol cell-repellent back-filling, enabling cell confinement in lanes as narrow as 5 μm. Cells patterned on narrow (5 and 10 μm) lanes showed preferred nucleus orientation following the patterning direction. These cells also showed high nucleus aspect ratio but constrained nucleus spreading. On the other hand, cells on wide (20 μm and above) lanes showed random nucleus orientation and cell and nucleus sizes similar to those on unpatterned controls. All these changes were generally maintained with or without RA. Confining cells on narrow (5 and 10 μm) lanes, even without RA, significantly enhanced neurite extension relative to unpatterned control, which was further stimulated by RA. Interestingly, cell patterning on 5 and 10 μm lanes without RA produced longer neurites relative to the RA treatment alone case. Our data on the potential interplay between microscale physical cell confinement and RA-soluble stimulation may provide a new, integrative insight on how to trigger neurite/axon formation for neuronal regenerative medicine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4592-4598 |
| Number of pages | 7 |
| Journal | Acta Biomaterialia |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2013 |
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Keywords
- Cell and nucleus orientation
- Cell micropatterning
- Neurite outgrowth
- Neuronal regenerative medicine
- Retinoic acid
ASJC Scopus subject areas
- Biotechnology
- Biomaterials
- Biochemistry
- Biomedical Engineering
- Molecular Biology
Cite this
Micropatterning-retinoic acid co-control of neuronal cell morphology and neurite outgrowth. / Poudel, Ishwari; Lee, Jeong Soon; Tan, Li; Lim, Jung Y.
In: Acta Biomaterialia, Vol. 9, No. 1, 01.01.2013, p. 4592-4598.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Micropatterning-retinoic acid co-control of neuronal cell morphology and neurite outgrowth
AU - Poudel, Ishwari
AU - Lee, Jeong Soon
AU - Tan, Li
AU - Lim, Jung Y
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Creating physical-biochemical superposed microenvironments optimal for stimulating neurite outgrowth would be beneficial for neuronal regenerative medicine. We investigated potential co-regulatory effects of cell micropatterning and retinoic acid (RA) soluble factor on neuronal cell morphology and neurite outgrowth. Human neuroblastoma (SH-SY5Y) cell patterning sensitivity could be enhanced by poly-l-lysine-g-polyethylene glycol cell-repellent back-filling, enabling cell confinement in lanes as narrow as 5 μm. Cells patterned on narrow (5 and 10 μm) lanes showed preferred nucleus orientation following the patterning direction. These cells also showed high nucleus aspect ratio but constrained nucleus spreading. On the other hand, cells on wide (20 μm and above) lanes showed random nucleus orientation and cell and nucleus sizes similar to those on unpatterned controls. All these changes were generally maintained with or without RA. Confining cells on narrow (5 and 10 μm) lanes, even without RA, significantly enhanced neurite extension relative to unpatterned control, which was further stimulated by RA. Interestingly, cell patterning on 5 and 10 μm lanes without RA produced longer neurites relative to the RA treatment alone case. Our data on the potential interplay between microscale physical cell confinement and RA-soluble stimulation may provide a new, integrative insight on how to trigger neurite/axon formation for neuronal regenerative medicine.
AB - Creating physical-biochemical superposed microenvironments optimal for stimulating neurite outgrowth would be beneficial for neuronal regenerative medicine. We investigated potential co-regulatory effects of cell micropatterning and retinoic acid (RA) soluble factor on neuronal cell morphology and neurite outgrowth. Human neuroblastoma (SH-SY5Y) cell patterning sensitivity could be enhanced by poly-l-lysine-g-polyethylene glycol cell-repellent back-filling, enabling cell confinement in lanes as narrow as 5 μm. Cells patterned on narrow (5 and 10 μm) lanes showed preferred nucleus orientation following the patterning direction. These cells also showed high nucleus aspect ratio but constrained nucleus spreading. On the other hand, cells on wide (20 μm and above) lanes showed random nucleus orientation and cell and nucleus sizes similar to those on unpatterned controls. All these changes were generally maintained with or without RA. Confining cells on narrow (5 and 10 μm) lanes, even without RA, significantly enhanced neurite extension relative to unpatterned control, which was further stimulated by RA. Interestingly, cell patterning on 5 and 10 μm lanes without RA produced longer neurites relative to the RA treatment alone case. Our data on the potential interplay between microscale physical cell confinement and RA-soluble stimulation may provide a new, integrative insight on how to trigger neurite/axon formation for neuronal regenerative medicine.
KW - Cell and nucleus orientation
KW - Cell micropatterning
KW - Neurite outgrowth
KW - Neuronal regenerative medicine
KW - Retinoic acid
UR - http://www.scopus.com/inward/record.url?scp=84870238879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870238879&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2012.08.039
DO - 10.1016/j.actbio.2012.08.039
M3 - Article
C2 - 22939924
AN - SCOPUS:84870238879
VL - 9
SP - 4592
EP - 4598
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 1
ER -