Micropatterning-retinoic acid co-control of neuronal cell morphology and neurite outgrowth

Ishwari Poudel, Jeong Soon Lee, Li Tan, Jung Y Lim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Creating physical-biochemical superposed microenvironments optimal for stimulating neurite outgrowth would be beneficial for neuronal regenerative medicine. We investigated potential co-regulatory effects of cell micropatterning and retinoic acid (RA) soluble factor on neuronal cell morphology and neurite outgrowth. Human neuroblastoma (SH-SY5Y) cell patterning sensitivity could be enhanced by poly-l-lysine-g-polyethylene glycol cell-repellent back-filling, enabling cell confinement in lanes as narrow as 5 μm. Cells patterned on narrow (5 and 10 μm) lanes showed preferred nucleus orientation following the patterning direction. These cells also showed high nucleus aspect ratio but constrained nucleus spreading. On the other hand, cells on wide (20 μm and above) lanes showed random nucleus orientation and cell and nucleus sizes similar to those on unpatterned controls. All these changes were generally maintained with or without RA. Confining cells on narrow (5 and 10 μm) lanes, even without RA, significantly enhanced neurite extension relative to unpatterned control, which was further stimulated by RA. Interestingly, cell patterning on 5 and 10 μm lanes without RA produced longer neurites relative to the RA treatment alone case. Our data on the potential interplay between microscale physical cell confinement and RA-soluble stimulation may provide a new, integrative insight on how to trigger neurite/axon formation for neuronal regenerative medicine.

Original languageEnglish (US)
Pages (from-to)4592-4598
Number of pages7
JournalActa Biomaterialia
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Tretinoin
Acids
Neurites
Regenerative Medicine
Neuronal Outgrowth
Cell Nucleus Size
Polyethylene glycols
Lysine
Aspect ratio
Neuroblastoma
Axons

Keywords

  • Cell and nucleus orientation
  • Cell micropatterning
  • Neurite outgrowth
  • Neuronal regenerative medicine
  • Retinoic acid

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Micropatterning-retinoic acid co-control of neuronal cell morphology and neurite outgrowth. / Poudel, Ishwari; Lee, Jeong Soon; Tan, Li; Lim, Jung Y.

In: Acta Biomaterialia, Vol. 9, No. 1, 01.01.2013, p. 4592-4598.

Research output: Contribution to journalArticle

Poudel, Ishwari ; Lee, Jeong Soon ; Tan, Li ; Lim, Jung Y. / Micropatterning-retinoic acid co-control of neuronal cell morphology and neurite outgrowth. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 1. pp. 4592-4598.
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