Microfluidic system for studying the interaction of nanoparticles and microparticles with cells

Omid C. Farokhzad, Ali Khademhosseini, Sangyong Jon, Aurelia Hermmann, Jianjun Cheng, Curtis Chin, Alice Kiselyuk, Benjamin Teply, George Eng, Robert Langer

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Nanoparticles and microparticles have many potential biomedical applications ranging from imaging to drug delivery. Therefore, in vitro systems that can analyze and optimize the interaction of such particles with cells may be beneficial. Here, we report a microfluidic system that can be used to study these interactions. As a model system, we evaluated the interaction of polymeric nanoparticles and microparticles and similar particles conjugated to aptamers that recognize the transmembrane prostate specific membrane antigen (PSMA), with cells seeded in microchannels. The binding of particles to cells that expressed or did not express the PSMA (LNCaP or PCS, respectively) were evaluated with respect to changes in fluid shear stress, PSMA expression on target cells, and particle size. Nanoparticle-aptamer bioconjugates selectively adhered to LNCaP but not PCS cells at static and low shear (<1 dyn/cm2) but not higher shear (∼4.5 dyn/cm2) conditions. Control nanoparticles and microparticles lacking aptamers and microparticle-aptamer bioconjugates did not adhere to LNCaP cells, even under very low shear conditions (∼0.28 dyn/cm2). These results demonstrate that the interaction of particles with cells can be studied under controlled conditions, which may aid in the engineering of desired particle characteristics. The scalability, low cost, reproducibiliry, and high-throughput capability of this technology is potentially beneficial to examining and optimizing a wide array of cell-particle systems prior to in vivo experiments.

Original languageEnglish (US)
Pages (from-to)5453-5459
Number of pages7
JournalAnalytical chemistry
Volume77
Issue number17
DOIs
StatePublished - Sep 1 2005

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Microfluidics
Nanoparticles
Microchannels
Drug delivery
Scalability
Shear stress
Particle size
Throughput
Imaging techniques
Fluids
human glutamate carboxypeptidase II
Costs
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Farokhzad, O. C., Khademhosseini, A., Jon, S., Hermmann, A., Cheng, J., Chin, C., ... Langer, R. (2005). Microfluidic system for studying the interaction of nanoparticles and microparticles with cells. Analytical chemistry, 77(17), 5453-5459. https://doi.org/10.1021/ac050312q

Microfluidic system for studying the interaction of nanoparticles and microparticles with cells. / Farokhzad, Omid C.; Khademhosseini, Ali; Jon, Sangyong; Hermmann, Aurelia; Cheng, Jianjun; Chin, Curtis; Kiselyuk, Alice; Teply, Benjamin; Eng, George; Langer, Robert.

In: Analytical chemistry, Vol. 77, No. 17, 01.09.2005, p. 5453-5459.

Research output: Contribution to journalArticle

Farokhzad, OC, Khademhosseini, A, Jon, S, Hermmann, A, Cheng, J, Chin, C, Kiselyuk, A, Teply, B, Eng, G & Langer, R 2005, 'Microfluidic system for studying the interaction of nanoparticles and microparticles with cells', Analytical chemistry, vol. 77, no. 17, pp. 5453-5459. https://doi.org/10.1021/ac050312q
Farokhzad OC, Khademhosseini A, Jon S, Hermmann A, Cheng J, Chin C et al. Microfluidic system for studying the interaction of nanoparticles and microparticles with cells. Analytical chemistry. 2005 Sep 1;77(17):5453-5459. https://doi.org/10.1021/ac050312q
Farokhzad, Omid C. ; Khademhosseini, Ali ; Jon, Sangyong ; Hermmann, Aurelia ; Cheng, Jianjun ; Chin, Curtis ; Kiselyuk, Alice ; Teply, Benjamin ; Eng, George ; Langer, Robert. / Microfluidic system for studying the interaction of nanoparticles and microparticles with cells. In: Analytical chemistry. 2005 ; Vol. 77, No. 17. pp. 5453-5459.
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