Microfluidic Device for Localized Electroporation

Justin Brooks, Arian Jaberi, Ruiguo Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells yet results in variable levels of viability and transfection efficiency across the population. Localized electroporation is an alternative that can be administered on a similar scale but results in much more consistent with higher quality transfection and higher cell viability. This chapter discusses the creation and use of a simple and cost-effective device using porous membrane for performing localized electroporation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages91-97
Number of pages7
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2050
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Lab-On-A-Chip Devices
Electroporation
Transfection
Cell Survival
Costs and Cost Analysis
Equipment and Supplies
Membranes

Keywords

  • Cell transfection
  • Lab-on-a-chip
  • Localized electroporation
  • Microfluidic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Brooks, J., Jaberi, A., & Yang, R. (2020). Microfluidic Device for Localized Electroporation. In Methods in Molecular Biology (pp. 91-97). (Methods in Molecular Biology; Vol. 2050). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9740-4_10

Microfluidic Device for Localized Electroporation. / Brooks, Justin; Jaberi, Arian; Yang, Ruiguo.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 91-97 (Methods in Molecular Biology; Vol. 2050).

Research output: Chapter in Book/Report/Conference proceedingChapter

Brooks, J, Jaberi, A & Yang, R 2020, Microfluidic Device for Localized Electroporation. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2050, Humana Press Inc., pp. 91-97. https://doi.org/10.1007/978-1-4939-9740-4_10
Brooks J, Jaberi A, Yang R. Microfluidic Device for Localized Electroporation. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 91-97. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9740-4_10
Brooks, Justin ; Jaberi, Arian ; Yang, Ruiguo. / Microfluidic Device for Localized Electroporation. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 91-97 (Methods in Molecular Biology).
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