Microfluidic based strip assay for surface bound inhibitors in spinal cord injury

Behrad Vahidi, Jeong Won Park, Hyung Joon Kim, Noo Li Jeon

Research output: Contribution to conferencePaper

Abstract

This paper describes a novel microfluidic-based assay for Spinal Cord Injury (SCI) research. Conventional methods such as neurite outgrowth and strip assays can not recapitulate the organized structure of the spinal cord and thus poorly simulate the injury microenvironment [1]. In SCI, the cell bodies are often located away from the immediate lesion, while the damaged and regenerating axons are exposed to the inhibitory milieu of the scar tissue. We have used combination of micropatterning and microfluidics to selectively place high purity dissociated CNS neurons on favorable substrate (i.e. PLL) but allow only axons to interact with permissive and inhibitory substrates (i.e. CSPG) presented in alternating strips. We show that on patterned surfaces, axons were confined on permissive lanes and consistently avoided inhibitory strips. This approach is applicable and amenable for high throughput screening and maybe used to study the direct effects of pharmaceuticals that might suppress inhibitors of neuronal extension and to provide valuable insight into the underlying mechanism of neurite outgrowth inhibition by these compounds.

Original languageEnglish (US)
Pages1894-1896
Number of pages3
StatePublished - Jan 1 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

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Keywords

  • Aggrecan
  • Cortical neuron
  • Micromolding in capillaries

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Vahidi, B., Park, J. W., Kim, H. J., & Jeon, N. L. (2008). Microfluidic based strip assay for surface bound inhibitors in spinal cord injury. 1894-1896. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.