Microbiota of the Major South Atlantic Reef Building Coral Mussismilia

Samodha C Fernando, Jia Wang, Kimberly Sparling, Gizele D. Garcia, Ronaldo B. Francini-Filho, Rodrigo L. de Moura, Rodolfo Paranhos, Fabiano L. Thompson, Janelle R. Thompson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Brazilian endemic scleractinian corals, genus Mussismilia, are among the main reef builders of the South Atlantic and are threatened by accelerating rates of disease. To better understand how holobiont microbial populations interact with corals during health and disease and to evaluate whether selective pressures in the holobiont or neutral assembly shape microbial composition, we have examined the microbiota structure of Mussismilia corals according to coral lineage, environment, and disease/health status. Microbiota of three Mussismilia species (Mussismilia harttii, Mussismilia hispida, and Mussismilia braziliensis) was compared using 16S rRNA pyrosequencing and clone library analysis of coral fragments. Analysis of biological triplicates per Mussismilia species and reef site allowed assessment of variability among Mussismilia species and between sites for M. braziliensis. From 173,487 V6 sequences, 6,733 coral- and 1,052 water-associated operational taxonomic units (OTUs) were observed. M. braziliensis microbiota was more similar across reefs than to other Mussismilia species microbiota from the same reef. Highly prevalent OTUs were more significantly structured by coral lineage and were enriched in Alpha- and Gammaproteobacteria. Bacterial OTUs from healthy corals were recovered from a M. braziliensis skeleton sample at twice the frequency of recovery from water or a diseased coral suggesting the skeleton is a significant habitat for microbial populations in the holobiont. Diseased corals were enriched with pathogens and opportunists (Vibrios, Bacteroidetes, Thalassomonas, and SRB). Our study examines for the first time intra- and inter-specific variability of microbiota across the genus Mussismilia. Changes in microbiota may be useful indicators of coral health and thus be a valuable tool for coral reef management and conservation.

Original languageEnglish (US)
Pages (from-to)267-280
Number of pages14
JournalMicrobial Ecology
Volume69
Issue number2
DOIs
StatePublished - Jan 1 2014

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corals
reefs
coral
reef
skeleton
microbiome
health and disease
gamma-Proteobacteria
Vibrio
health status
interspecific variation
coral reefs
coral reef
clone
water
ribosomal RNA
pathogen
clones
pathogens
habitats

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

Fernando, S. C., Wang, J., Sparling, K., Garcia, G. D., Francini-Filho, R. B., de Moura, R. L., ... Thompson, J. R. (2014). Microbiota of the Major South Atlantic Reef Building Coral Mussismilia. Microbial Ecology, 69(2), 267-280. https://doi.org/10.1007/s00248-014-0474-6

Microbiota of the Major South Atlantic Reef Building Coral Mussismilia. / Fernando, Samodha C; Wang, Jia; Sparling, Kimberly; Garcia, Gizele D.; Francini-Filho, Ronaldo B.; de Moura, Rodrigo L.; Paranhos, Rodolfo; Thompson, Fabiano L.; Thompson, Janelle R.

In: Microbial Ecology, Vol. 69, No. 2, 01.01.2014, p. 267-280.

Research output: Contribution to journalArticle

Fernando, SC, Wang, J, Sparling, K, Garcia, GD, Francini-Filho, RB, de Moura, RL, Paranhos, R, Thompson, FL & Thompson, JR 2014, 'Microbiota of the Major South Atlantic Reef Building Coral Mussismilia', Microbial Ecology, vol. 69, no. 2, pp. 267-280. https://doi.org/10.1007/s00248-014-0474-6
Fernando SC, Wang J, Sparling K, Garcia GD, Francini-Filho RB, de Moura RL et al. Microbiota of the Major South Atlantic Reef Building Coral Mussismilia. Microbial Ecology. 2014 Jan 1;69(2):267-280. https://doi.org/10.1007/s00248-014-0474-6
Fernando, Samodha C ; Wang, Jia ; Sparling, Kimberly ; Garcia, Gizele D. ; Francini-Filho, Ronaldo B. ; de Moura, Rodrigo L. ; Paranhos, Rodolfo ; Thompson, Fabiano L. ; Thompson, Janelle R. / Microbiota of the Major South Atlantic Reef Building Coral Mussismilia. In: Microbial Ecology. 2014 ; Vol. 69, No. 2. pp. 267-280.
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