Microbiology of the oil fly, Helaeomyia petrolei

Dana R. Kadavy, Bradley Plantz, Christopher A. Shaw, Jill Myatt, Tyler A. Kokjohn, Kenneth Nickerson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Helaeomyia petrolei larvae isolated from the asphalt seeps of Rancho La Brea in Los Angeles, Calif., were examined for microbial gut contents. Standard counts on Luria-Bertani, MacConkey, and blood agar plates indicated ca. 2 x 105 heterotrophic bacteria per larva. The culturable bacteria represented 15 to 20% of the total population as determined by acridine orange staining. The gut itself contained large amounts of the oil, had no observable ceca, and maintained a slightly acidic pH of 6.3 to 6.5. Despite the ingestion of large amounts of potentially toxic asphalt by the larvae, their guts sustained the growth of 100 to 1,000 times more bacteria than did free oil. All of the bacteria isolated were nonsporeformers and gram negative. Fourteen isolates were chosen based on representative colony morphologies and were identified by using the Enterotube II and API 20E systems and fatty acid analysis. Of the 14 isolates, 9 were identified as Providencia rettgeri and 3 were likely Acinetobacter isolates. No evidence was found that the isolates grew on or derived nutrients from the asphalt itself or that they played an essential role in insect development. Regardless, any bacteria found in the oil fly larval gut are likely to exhibit pronounced solvent tolerance and may be a future source of industrially useful, solvent-tolerant enzymes.

Original languageEnglish (US)
Pages (from-to)1477-1482
Number of pages6
JournalApplied and environmental microbiology
Volume65
Issue number4
StatePublished - Apr 1 1999

Fingerprint

asphalt
microbiology
Microbiology
Diptera
bitumen
Oils
Bacteria
oils
bacterium
oil
bacteria
Larva
digestive system
larva
larvae
Providencia rettgeri
Providencia
insect development
acridine orange
Acinetobacter

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Kadavy, D. R., Plantz, B., Shaw, C. A., Myatt, J., Kokjohn, T. A., & Nickerson, K. (1999). Microbiology of the oil fly, Helaeomyia petrolei. Applied and environmental microbiology, 65(4), 1477-1482.

Microbiology of the oil fly, Helaeomyia petrolei. / Kadavy, Dana R.; Plantz, Bradley; Shaw, Christopher A.; Myatt, Jill; Kokjohn, Tyler A.; Nickerson, Kenneth.

In: Applied and environmental microbiology, Vol. 65, No. 4, 01.04.1999, p. 1477-1482.

Research output: Contribution to journalArticle

Kadavy, DR, Plantz, B, Shaw, CA, Myatt, J, Kokjohn, TA & Nickerson, K 1999, 'Microbiology of the oil fly, Helaeomyia petrolei', Applied and environmental microbiology, vol. 65, no. 4, pp. 1477-1482.
Kadavy DR, Plantz B, Shaw CA, Myatt J, Kokjohn TA, Nickerson K. Microbiology of the oil fly, Helaeomyia petrolei. Applied and environmental microbiology. 1999 Apr 1;65(4):1477-1482.
Kadavy, Dana R. ; Plantz, Bradley ; Shaw, Christopher A. ; Myatt, Jill ; Kokjohn, Tyler A. ; Nickerson, Kenneth. / Microbiology of the oil fly, Helaeomyia petrolei. In: Applied and environmental microbiology. 1999 ; Vol. 65, No. 4. pp. 1477-1482.
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