Microbial communities in the rhizosphere and the root of lettuce as affected by Salmonella-contaminated irrigation water

Yuping Zhang, Christian Jewett, John Gilley, Shannon L Bartelt-Hunt, Daniel D Snow, Laurie Hodges, Xu Li

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Reclaimed wastewater is increasingly used as a source of irrigation water in croplands. The enteric pathogens in reclaimed wastewater may accumulate in soil and plants and cause food safety concerns. The objective of this study was to determine the effects of irrigation water containing Salmonella on the microbial communities in the rhizosphere and in the root of lettuce. The effects were also examined with three variables (soil texture, lettuce cultivar and harvest time) in a factorial design. Analyses of the 16S rRNA gene sequences show that the microbial communities in the root were significantly different from those in the rhizosphere, although ∼80% of the microbes in the root originated from the rhizosphere. Salmonella in irrigation water significantly altered the structure of the microbial community in the rhizosphere, but not in the root. Salmonella internalized in lettuce root was observed when contaminated water was used for irrigation. Compared to lettuce cultivar and harvest time, soil texture played a more significant role in shaping the bacterial communities in the rhizosphere and in the root. Results from this study could advance understanding about the long-term impact of reclaimed wastewater as a source of irrigation water on the microbiota associated with leafy green vegetables.

Original languageEnglish (US)
JournalFEMS microbiology ecology
Volume94
Issue number9
DOIs
StatePublished - Sep 1 2018

Fingerprint

Lettuce
Rhizosphere
Salmonella
rhizosphere
microbial community
irrigation
Waste Water
Water
Soil
soil texture
wastewater
water
cultivar
Food Safety
Microbiota
rRNA Genes
food safety
Vegetables
salmonella
pathogen

Keywords

  • Salmonella
  • food safety
  • lettuce
  • reclaimed wastewater
  • rhizosphere
  • root

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Microbial communities in the rhizosphere and the root of lettuce as affected by Salmonella-contaminated irrigation water. / Zhang, Yuping; Jewett, Christian; Gilley, John; Bartelt-Hunt, Shannon L; Snow, Daniel D; Hodges, Laurie; Li, Xu.

In: FEMS microbiology ecology, Vol. 94, No. 9, 01.09.2018.

Research output: Contribution to journalArticle

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