Mice with a fluorescent marker for interleukin 2 gene activation

Mayumi Naramura, Ren Ju Hu, Hua Gu

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Production of interleukin (IL)-2 by T lymphocytes is one of the earliest events during immune response. A mutant mouse strain was generated by replacing the IL-2 gene with a cDNA encoding green fluorescent protein (GFP). In this model, GFP fluorescence is readily detectable upon T cell activation and is mostly coexpressed with IL-2 at the single cell level. Thus, individual activated T cells can express the IL-2 gene biallelically. Upon stimulation through the T cell antigen receptor, CD4+ cells separate into distinct GFP+ and GFP- populations, both of which are capable of differentiating into either Th1 or Th2 effectors. These mice allow noninvasive detection of IL-2 production by single cells and analysis of the subsequent differentiative fate of these cells as an immune response develops.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalImmunity
Volume9
Issue number2
DOIs
Publication statusPublished - Aug 1998

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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