MICAL-L1 links EHD1 to tubular recycling endosomes and regulates receptor recycling

Mahak Sharma, Sai Srinivas Panapakkam Giridharan, Juliati Rahajeng, Naava Naslavsky, Steven H Caplan

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Endocytic recycling of receptors and lipids occurs via a complex network of tubular and vesicular membranes. EHD1 is a key regulator of endocytosis and associates with tubular membranes to facilitate recycling. Although EHD proteins tubulate membranes in vitro, EHD1 primarily associates with preexisting tubules in vivo. How EHD1 is recruited to these tubular endosomes remains unclear. We have determined that the Rab8-interacting protein, MICAL-L1, associates with EHD1, with both proteins colocalizing to long tubular membranes, in vitro and in live cells. MICAL-L1 is a largely uncharacterized member of the MICAL-family of proteins that uniquely contains two asparagine-proline-phenylalanine motifs, sequences that typically interact with EH-domains. Our data show that the MICAL-L1 C-terminal coiled-coil region is necessary and sufficient for its localization to tubular membranes. Moreover, we provide unexpected evidence that endogenous MICAL-L1 can link both EHD1 and Rab8a to these structures, as its depletion leads to loss of the EHD1-Rab8a interaction and the absence of both of these proteins from the membrane tubules. Finally, we demonstrate that MICAL-L1 is essential for efficient endocytic recycling. These data implicate MICAL-L1 as an unusual type of Rab effector that regulates endocytic recycling by recruiting and linking EHD1 and Rab8a on membrane tubules.

Original languageEnglish (US)
Pages (from-to)5181-5194
Number of pages14
JournalMolecular biology of the cell
Volume20
Issue number24
DOIs
StatePublished - Dec 1 2009

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Endosomes
Membranes
Membrane Proteins
Proteins
Asparagine
Recycling
Endocytosis
Phenylalanine
Proline
Lipids
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

MICAL-L1 links EHD1 to tubular recycling endosomes and regulates receptor recycling. / Sharma, Mahak; Giridharan, Sai Srinivas Panapakkam; Rahajeng, Juliati; Naslavsky, Naava; Caplan, Steven H.

In: Molecular biology of the cell, Vol. 20, No. 24, 01.12.2009, p. 5181-5194.

Research output: Contribution to journalArticle

Sharma, Mahak ; Giridharan, Sai Srinivas Panapakkam ; Rahajeng, Juliati ; Naslavsky, Naava ; Caplan, Steven H. / MICAL-L1 links EHD1 to tubular recycling endosomes and regulates receptor recycling. In: Molecular biology of the cell. 2009 ; Vol. 20, No. 24. pp. 5181-5194.
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