MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium

Shuwei Xie, Trey Farmer, Naava Naslavsky, Steve Caplan

Research output: Contribution to journalArticle

Abstract

The endocytic protein EHD1 plays an important role in ciliogenesis by facilitating fusion of the ciliary vesicle and removal of CP110 (also known as CCP110) from the mother centriole, as well as removal of Cep215 (also known as CDK5RAP2) from centrioles to permit disengagement and duplication. However, the mechanism of its centrosomal recruitment remains unknown. Here, we address the role of the EHD1 interaction partner MICAL-L1 in ciliogenesis. MICAL-L1 knockdown impairs ciliogenesis in a similar manner to EHD1 knockdown, and MICAL-L1 localizes to cilia and centrosomes in both ciliated and non-ciliated cells. Consistent with EHD1 function, MICAL-L1-depletion prevents CP110 removal from the mother centriole. Moreover, upon MICAL-L1-depletion, EHD1 fails to localize to basal bodies. Since MICAL-L1 localizes to the centrosome even in non-ciliated cells, we hypothesized that it might be anchored to the centrosome via an interaction with centrosomal proteins. By performing mass spectrometry, we identified several tubulins as potential MICAL-L1 interaction partners, and found a direct interaction between MICAL-L1 and both α-tubulin-β-tubulin heterodimers and γ-tubulin. Our data support the notion that a pool of centriolar γ-tubulin and/or α-tubulin-β-tubulin heterodimers anchor MICAL-L1 to the centriole, where it might recruit EHD1 to promote ciliogenesis.

Original languageEnglish (US)
JournalJournal of cell science
Volume132
Issue number22
DOIs
StatePublished - Nov 14 2019

Fingerprint

Cilia
Tubulin
Centrioles
Centrosome
Basal Bodies
Mass Spectrometry
Proteins

Keywords

  • Centriole
  • Cilia
  • Ciliogenesis
  • EHD1
  • MICAL-L1
  • Tubulin

ASJC Scopus subject areas

  • Cell Biology

Cite this

MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium. / Xie, Shuwei; Farmer, Trey; Naslavsky, Naava; Caplan, Steve.

In: Journal of cell science, Vol. 132, No. 22, 14.11.2019.

Research output: Contribution to journalArticle

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