Methylation patterns of histone H3 Lys 4, Lys 9 and Lys 27 in transcriptionally active and inactive Arabidopsis genes and in atx1 mutants

Raul Alvarez-Venegas, Zoya Avramova

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Covalent modifications of histone-tail amino acid residues communicate information via a specific 'histone code'. Here, we report histone H3-tail lysine methylation profiles of several Arabidopsis genes in correlation with their transcriptional activity and the input of the epigenetic factor ARABIDOPSIS HOMOLOG OF TRITHORAX (ATX1) at ATX1-regulated loci. By chromatin immunoprecipitation (ChIP) assays, we compared modification patterns of a constitutively expressed housekeeping gene, of a tissue-specific gene, and among genes that differed in degrees of transcriptional activity. Our results suggest that the di-methylated isoform of histone H3-lysine4 (m2K4/H3) provide a general mark for gene-related sequences distinguishing them from non-transcribed regions. Lys-4 (K4/H3), lys-9 (K9/H3) and lys-27 (K27/H3) nucleosome methylation patterns of plant genes may be gene-, tissue- or development-regulated. Absence of nucleosomes from the LTP-promotor was not sufficient to provoke robust transcription in mutant atx1-leaf chromatin, suggesting that the mechanism repositioning nucleosomes at transition to flowering functioned independently of ATX1.

Original languageEnglish (US)
Pages (from-to)5199-5207
Number of pages9
JournalNucleic acids research
Volume33
Issue number16
DOIs
StatePublished - Nov 1 2005

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Arabidopsis
Histones
Methylation
Nucleosomes
Histone Code
Genes
Plant Genes
Chromatin Immunoprecipitation
Essential Genes
Epigenomics
Lysine
Chromatin
Protein Isoforms
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Methylation patterns of histone H3 Lys 4, Lys 9 and Lys 27 in transcriptionally active and inactive Arabidopsis genes and in atx1 mutants. / Alvarez-Venegas, Raul; Avramova, Zoya.

In: Nucleic acids research, Vol. 33, No. 16, 01.11.2005, p. 5199-5207.

Research output: Contribution to journalArticle

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