Methylation as a crucial step in plant microRNA biogenesis

Bin Yu, Zhiyong Yang, Junjie Li, Svetlana Minakhina, Maocheng Yang, Richard W. Padgett, Ruth Steward, Xuemei Chen

Research output: Contribution to journalArticle

621 Citations (Scopus)

Abstract

Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2′-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.

Original languageEnglish (US)
Pages (from-to)932-935
Number of pages4
JournalScience
Volume307
Issue number5711
DOIs
StatePublished - Feb 11 2005

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MicroRNAs
Methylation
Ribose
Protein Methyltransferases
Ribosomal RNA
Ribosomes
Small Interfering RNA
Nucleotides
Serum

ASJC Scopus subject areas

  • General

Cite this

Yu, B., Yang, Z., Li, J., Minakhina, S., Yang, M., Padgett, R. W., ... Chen, X. (2005). Methylation as a crucial step in plant microRNA biogenesis. Science, 307(5711), 932-935. https://doi.org/10.1126/science.1107130

Methylation as a crucial step in plant microRNA biogenesis. / Yu, Bin; Yang, Zhiyong; Li, Junjie; Minakhina, Svetlana; Yang, Maocheng; Padgett, Richard W.; Steward, Ruth; Chen, Xuemei.

In: Science, Vol. 307, No. 5711, 11.02.2005, p. 932-935.

Research output: Contribution to journalArticle

Yu, B, Yang, Z, Li, J, Minakhina, S, Yang, M, Padgett, RW, Steward, R & Chen, X 2005, 'Methylation as a crucial step in plant microRNA biogenesis', Science, vol. 307, no. 5711, pp. 932-935. https://doi.org/10.1126/science.1107130
Yu B, Yang Z, Li J, Minakhina S, Yang M, Padgett RW et al. Methylation as a crucial step in plant microRNA biogenesis. Science. 2005 Feb 11;307(5711):932-935. https://doi.org/10.1126/science.1107130
Yu, Bin ; Yang, Zhiyong ; Li, Junjie ; Minakhina, Svetlana ; Yang, Maocheng ; Padgett, Richard W. ; Steward, Ruth ; Chen, Xuemei. / Methylation as a crucial step in plant microRNA biogenesis. In: Science. 2005 ; Vol. 307, No. 5711. pp. 932-935.
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