Methods to monitor Fatty Acid transport proceeding through vectorial acylation.

Elsa Arias-Barrau, Concetta C DiRusso, Paul N Black

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The process of fatty acid transport across the plasma membrane occurs by several mechanisms that involve distinct membrane-bound and membrane-associated proteins and enzymes. Among these are the fatty acid transport proteins (FATP) and long-chain acyl CoA synthetases (Acsl). Previous studies in yeast and adipocytes have shown FATP and Acsl form a physical complex at the plasma membrane and are required for fatty acid transport, which proceeds through a coupled process-linking transport with metabolic activation termed vectorial acylation. At present, six isoforms of FATP and five isoforms of ACSL have been identified in mice and man. In addition, there are a number of splice variants of different FATP and Acsl isoforms. The different FATP and Acsl isoforms have distinct tissue expression profiles and different cellular locations suggesting they function in the channeling of fatty acids into discrete metabolic pools. The concerted activity of these proteins is proposed to allow cells to discriminate different classes of fatty acids and provides the mechanistic basis underpinning the selectivity and specificity of the fatty acid transport process.

Original languageEnglish (US)
Pages (from-to)233-249
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume580
StatePublished - Jan 1 2009

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Fatty Acid Transport Proteins
Acylation
Fatty Acids
Protein Isoforms
Cell Membrane
Coenzyme A Ligases
Acyl Coenzyme A
Adipocytes
Membrane Proteins
Yeasts
Membranes
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Methods to monitor Fatty Acid transport proceeding through vectorial acylation. / Arias-Barrau, Elsa; DiRusso, Concetta C; Black, Paul N.

In: Methods in molecular biology (Clifton, N.J.), Vol. 580, 01.01.2009, p. 233-249.

Research output: Contribution to journalArticle

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