Methods to detect apoptotic-like cell death in filamentous fungi.

Camile P. Semighini, Steven D. Harris

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Fungi are capable of undergoing apoptotic-like cell death, and display many of the characteristic features of apoptosis observed in multicellular organisms. These features include nuclear condensation, DNA fragmentation, translocation of phosphatidylserine from the cytoplasmic to the extracellular side of the plasma membrane, and increased levels of reactive oxygen species (ROS). Several assays can be used to detect apoptotic cells, and here we describe adaptations of assays such as TUNEL, Annexin V, and Evan's Blue for the investigation of apoptotic-like cell death in fungal hyphae. We also present approaches for monitoring nuclear condensation and production of ROS.

Original languageEnglish (US)
Pages (from-to)269-279
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume638
DOIs
StatePublished - 2010

Fingerprint

Reactive Oxygen Species
Fungi
Cell Death
Evans Blue
Hyphae
Annexin A5
Phosphatidylserines
In Situ Nick-End Labeling
DNA Fragmentation
Cell Membrane
Apoptosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Methods to detect apoptotic-like cell death in filamentous fungi. / Semighini, Camile P.; Harris, Steven D.

In: Methods in molecular biology (Clifton, N.J.), Vol. 638, 2010, p. 269-279.

Research output: Contribution to journalArticle

Semighini, Camile P. ; Harris, Steven D. / Methods to detect apoptotic-like cell death in filamentous fungi. In: Methods in molecular biology (Clifton, N.J.). 2010 ; Vol. 638. pp. 269-279.
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