Methods for isolation and identification of nanoparticle-containing subcellular compartments

Ari Nowacek, Irena Kadiu, JoEllyn M McMillan, Howard Eliot Gendelman

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Nanoparticle-based drug delivery systems have considerable potential for improvement of drug stability, bioavailability, and reduced dosing frequency. Important technological advantages of nanoparticles include high carrier capacity across biological membranes and controlled drug release. Ultimately, success of nanodelivery systems depends on toxicologic issues associated with the understanding of the fate of nanocarriers and their polymeric constituents within the targeted cells. Here we describe a method for determining subcellular distribution of nanoparticles by isolation and identification of organelles that come in direct contact with these structures.

Original languageEnglish (US)
Title of host publicationCellular and Subcellular Nanotechnology
Subtitle of host publicationMethods and Protocols
EditorsVolkmar Weissig, Mark Olsen, Tamer Elbayoumi
Pages47-55
Number of pages9
DOIs
StatePublished - May 8 2013

Publication series

NameMethods in Molecular Biology
Volume991
ISSN (Print)1064-3745

Fingerprint

Nanoparticles
Drug Stability
Drug Delivery Systems
Organelles
Biological Availability
Membranes
Drug Liberation

Keywords

  • Drug delivery
  • Endosome
  • Macrophage
  • Nanoparticle
  • Subcellular trafficking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nowacek, A., Kadiu, I., McMillan, J. M., & Gendelman, H. E. (2013). Methods for isolation and identification of nanoparticle-containing subcellular compartments. In V. Weissig, M. Olsen, & T. Elbayoumi (Eds.), Cellular and Subcellular Nanotechnology: Methods and Protocols (pp. 47-55). (Methods in Molecular Biology; Vol. 991). https://doi.org/10.1007/978-1-62703-336-7_6

Methods for isolation and identification of nanoparticle-containing subcellular compartments. / Nowacek, Ari; Kadiu, Irena; McMillan, JoEllyn M; Gendelman, Howard Eliot.

Cellular and Subcellular Nanotechnology: Methods and Protocols. ed. / Volkmar Weissig; Mark Olsen; Tamer Elbayoumi. 2013. p. 47-55 (Methods in Molecular Biology; Vol. 991).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nowacek, A, Kadiu, I, McMillan, JM & Gendelman, HE 2013, Methods for isolation and identification of nanoparticle-containing subcellular compartments. in V Weissig, M Olsen & T Elbayoumi (eds), Cellular and Subcellular Nanotechnology: Methods and Protocols. Methods in Molecular Biology, vol. 991, pp. 47-55. https://doi.org/10.1007/978-1-62703-336-7_6
Nowacek A, Kadiu I, McMillan JM, Gendelman HE. Methods for isolation and identification of nanoparticle-containing subcellular compartments. In Weissig V, Olsen M, Elbayoumi T, editors, Cellular and Subcellular Nanotechnology: Methods and Protocols. 2013. p. 47-55. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-336-7_6
Nowacek, Ari ; Kadiu, Irena ; McMillan, JoEllyn M ; Gendelman, Howard Eliot. / Methods for isolation and identification of nanoparticle-containing subcellular compartments. Cellular and Subcellular Nanotechnology: Methods and Protocols. editor / Volkmar Weissig ; Mark Olsen ; Tamer Elbayoumi. 2013. pp. 47-55 (Methods in Molecular Biology).
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