Methodologies for monitoring nanoparticle formation by self-assembly of DNA with poly(L-lysine)

Alan L. Parker, David Oupicky, Philip R. Dash, Leonard W. Seymour

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

DNA self-assembly with polycations produces nanoparticles suitable for gene delivery, although there is no standard methodology to measure particle formation and stability. Here we have compared three commonly used assays, namely, light scattering, inhibition of ethidium bromide fluorescence, and modified electrophoretic mobility of DNA. Analysis by light scattering and loss of ethidium bromide fluorescence both showed poly(L-lysine) (pLL)/DNA nanoparticles form over the lysine/phosphate ratio range 0.6-1.0, although retardation of DNA electrophoretic mobility commenced at lower lysine/phosphate ratios. This probably indicates that the first two assays monitor DNA collapse into particles, while the electrophoresis assay measures neutralization of the charge on DNA. Gel analysis of the complexes showed disproportionation during nanoparticle formation, probably reflecting cooperative binding of the polycation. The assays were used to examine stability of complexes to dilution in water and physiological salts. Whereas all pLL/DNA nanoparticles were stable to dilution in water, the presence of physiological salts provoked selective disruption of complexes based on low-molecular-weight pLL. Polyelectrolyte complexes for targeted application in vivo should therefore be based on high-molecular-weight polycations, or should be stabilized to prevent their dissociation under physiological salt conditions.

Original languageEnglish (US)
Pages (from-to)75-80
Number of pages6
JournalAnalytical Biochemistry
Volume302
Issue number1
DOIs
StatePublished - Mar 1 2002

Fingerprint

Nanoparticles
Self assembly
Lysine
Monitoring
DNA
Assays
Electrophoretic mobility
Ethidium
Salts
Light scattering
Dilution
Molecular Weight
Fluorescence
Molecular weight
Phosphates
Light
Water
Polyelectrolytes
Electrophoresis
Genes

Keywords

  • Condensation
  • Ethidium bromide
  • Gene therapy
  • Poly(L-lysine)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Methodologies for monitoring nanoparticle formation by self-assembly of DNA with poly(L-lysine). / Parker, Alan L.; Oupicky, David; Dash, Philip R.; Seymour, Leonard W.

In: Analytical Biochemistry, Vol. 302, No. 1, 01.03.2002, p. 75-80.

Research output: Contribution to journalArticle

Parker, Alan L. ; Oupicky, David ; Dash, Philip R. ; Seymour, Leonard W. / Methodologies for monitoring nanoparticle formation by self-assembly of DNA with poly(L-lysine). In: Analytical Biochemistry. 2002 ; Vol. 302, No. 1. pp. 75-80.
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