Method for attaining fennel (Foeniculum vulgare Mill.) seed oil fractions with different composition and antioxidant capacity

Andy Burkhardt, Henry Y. Sintim, Archana Gawde, Charles L. Cantrell, Tess Astatkie, Valtcho D. Zheljazkov, Vicki L Schlegel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fennel (Foeniculum vulgare Mill) is cultivated for its seeds and foliage, which contain essential oil. We hypothesized that the collection of fennel seed oil at different time points during the distillation process may result in fennel oil with distinct composition and bioactivity. We collected essential oil from fennel seed at ten different distillation times, DT (at 2, 7, 15, 30, 45, 75, 105, 135, 165, and 195. min). Estragole (42.8-84.4% of the total oil) was the main constituent throughout the distillation. (-)-α-Pinene (0.12-0.84%) and (R)-(+)-limonene (6.3-33.5%) were relatively high at initial DT and dropped significantly after the first DT. (-)-Fenchone (0.5-4.1%) showed a near linear decrease until approximately the 165. min DT. If high (-)-α-pinene and (R)-(+)-limonene oil is desirable, then the oil fraction needs to be captured early in the distillation, at 2. min. Oil with highest estragole concentration may be obtained by collecting the oil eluted between 30 and 45. min. DT had an effect on the oil antioxidant activity; the oil from the control (195. min distillation time) had the highest antioxidant capacity (481. μmol. Trolox. equivalents/g), whereas the oil fraction sampled at 45. min DT had the lowest capacity (148. μmol. Trolox. equivalents/g). This study demonstrated an effective method for obtaining fennel seed oil fractions with differential composition and antioxidant capacity.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalJournal of Applied Research on Medicinal and Aromatic Plants
Volume2
Issue number3
DOIs
StatePublished - Sep 1 2015

Fingerprint

Foeniculum
Foeniculum vulgare
fennel
seed oils
Seeds
Oils
Antioxidants
antioxidants
oils
distillation
Distillation
methyl chavicol
methodology
Volatile Oils
essential oils
seeds
antioxidant activity

Keywords

  • Antioxidant capacity
  • Composition
  • Essential oil content
  • Estragole
  • Foeniculum vulgare
  • Hydrodistillation

ASJC Scopus subject areas

  • Drug Discovery
  • Plant Science

Cite this

Method for attaining fennel (Foeniculum vulgare Mill.) seed oil fractions with different composition and antioxidant capacity. / Burkhardt, Andy; Sintim, Henry Y.; Gawde, Archana; Cantrell, Charles L.; Astatkie, Tess; Zheljazkov, Valtcho D.; Schlegel, Vicki L.

In: Journal of Applied Research on Medicinal and Aromatic Plants, Vol. 2, No. 3, 01.09.2015, p. 87-91.

Research output: Contribution to journalArticle

Burkhardt, Andy ; Sintim, Henry Y. ; Gawde, Archana ; Cantrell, Charles L. ; Astatkie, Tess ; Zheljazkov, Valtcho D. ; Schlegel, Vicki L. / Method for attaining fennel (Foeniculum vulgare Mill.) seed oil fractions with different composition and antioxidant capacity. In: Journal of Applied Research on Medicinal and Aromatic Plants. 2015 ; Vol. 2, No. 3. pp. 87-91.
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abstract = "Fennel (Foeniculum vulgare Mill) is cultivated for its seeds and foliage, which contain essential oil. We hypothesized that the collection of fennel seed oil at different time points during the distillation process may result in fennel oil with distinct composition and bioactivity. We collected essential oil from fennel seed at ten different distillation times, DT (at 2, 7, 15, 30, 45, 75, 105, 135, 165, and 195. min). Estragole (42.8-84.4{\%} of the total oil) was the main constituent throughout the distillation. (-)-α-Pinene (0.12-0.84{\%}) and (R)-(+)-limonene (6.3-33.5{\%}) were relatively high at initial DT and dropped significantly after the first DT. (-)-Fenchone (0.5-4.1{\%}) showed a near linear decrease until approximately the 165. min DT. If high (-)-α-pinene and (R)-(+)-limonene oil is desirable, then the oil fraction needs to be captured early in the distillation, at 2. min. Oil with highest estragole concentration may be obtained by collecting the oil eluted between 30 and 45. min. DT had an effect on the oil antioxidant activity; the oil from the control (195. min distillation time) had the highest antioxidant capacity (481. μmol. Trolox. equivalents/g), whereas the oil fraction sampled at 45. min DT had the lowest capacity (148. μmol. Trolox. equivalents/g). This study demonstrated an effective method for obtaining fennel seed oil fractions with differential composition and antioxidant capacity.",
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AU - Burkhardt, Andy

AU - Sintim, Henry Y.

AU - Gawde, Archana

AU - Cantrell, Charles L.

AU - Astatkie, Tess

AU - Zheljazkov, Valtcho D.

AU - Schlegel, Vicki L

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N2 - Fennel (Foeniculum vulgare Mill) is cultivated for its seeds and foliage, which contain essential oil. We hypothesized that the collection of fennel seed oil at different time points during the distillation process may result in fennel oil with distinct composition and bioactivity. We collected essential oil from fennel seed at ten different distillation times, DT (at 2, 7, 15, 30, 45, 75, 105, 135, 165, and 195. min). Estragole (42.8-84.4% of the total oil) was the main constituent throughout the distillation. (-)-α-Pinene (0.12-0.84%) and (R)-(+)-limonene (6.3-33.5%) were relatively high at initial DT and dropped significantly after the first DT. (-)-Fenchone (0.5-4.1%) showed a near linear decrease until approximately the 165. min DT. If high (-)-α-pinene and (R)-(+)-limonene oil is desirable, then the oil fraction needs to be captured early in the distillation, at 2. min. Oil with highest estragole concentration may be obtained by collecting the oil eluted between 30 and 45. min. DT had an effect on the oil antioxidant activity; the oil from the control (195. min distillation time) had the highest antioxidant capacity (481. μmol. Trolox. equivalents/g), whereas the oil fraction sampled at 45. min DT had the lowest capacity (148. μmol. Trolox. equivalents/g). This study demonstrated an effective method for obtaining fennel seed oil fractions with differential composition and antioxidant capacity.

AB - Fennel (Foeniculum vulgare Mill) is cultivated for its seeds and foliage, which contain essential oil. We hypothesized that the collection of fennel seed oil at different time points during the distillation process may result in fennel oil with distinct composition and bioactivity. We collected essential oil from fennel seed at ten different distillation times, DT (at 2, 7, 15, 30, 45, 75, 105, 135, 165, and 195. min). Estragole (42.8-84.4% of the total oil) was the main constituent throughout the distillation. (-)-α-Pinene (0.12-0.84%) and (R)-(+)-limonene (6.3-33.5%) were relatively high at initial DT and dropped significantly after the first DT. (-)-Fenchone (0.5-4.1%) showed a near linear decrease until approximately the 165. min DT. If high (-)-α-pinene and (R)-(+)-limonene oil is desirable, then the oil fraction needs to be captured early in the distillation, at 2. min. Oil with highest estragole concentration may be obtained by collecting the oil eluted between 30 and 45. min. DT had an effect on the oil antioxidant activity; the oil from the control (195. min distillation time) had the highest antioxidant capacity (481. μmol. Trolox. equivalents/g), whereas the oil fraction sampled at 45. min DT had the lowest capacity (148. μmol. Trolox. equivalents/g). This study demonstrated an effective method for obtaining fennel seed oil fractions with differential composition and antioxidant capacity.

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KW - Hydrodistillation

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