Metanephric osteopontin regulates nephrogenesis in vitro

Sharon A. Rogers, Babu J. Padanilam, Keith A. Hruska, Cecilia M. Giachelli, Marc R. Hammerman

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Renal expression of osteopontin is enhanced in the setting of acute ischemic injury. Because of the parallels that exist between recovery from renalischemia and renal development, we characterized the role that osteopontin plays during metanephrogenesis in the rat. Osteopontin mRNA is present in kidneys obtained from rat embryos as early as embryonic day 13 (E13). Immunohistochemical staining of metanephroi obtained from E16 rat embryos and metanephroi obtained from E13 embryos and cultured for 3 days in vitro demonstrated that osteopontin is expressed both in the developing nephron and in the ureteric bud. Addition of anti-osteopontin antibodies to metanephric organ cultures results in failure of the metanephrio blastema to undergo normal tubulogenesis. Addition of the arginine-glycine-aspartic acid- containing peptide, cyclo-RGDfV, or the anti-α(v)β3-integrin antibody, LM609, to cultures has a similar effect. These findings establish that osteopontin is produced within the rat metanephros during development in vivo and suggest that the binding of osteopontin to the α(v)β3-integrin is required for tubulogenesis to occur in vitro. Blastemal cells within metanephroi cultured in the presence of OP199 manifest increased apoptosis compared with controls. It is possible that osteopontin plays an important anti-apoptotic role during the process of metanephric blastema condensation that is a prerequisite for the formation of nephrons in vivo.

Original languageEnglish (US)
Pages (from-to)F469-F476
JournalAmerican Journal of Physiology - Renal Physiology
Volume272
Issue number4 41-4
StatePublished - Apr 1 1997

Fingerprint

Osteopontin
Embryonic Structures
Nephrons
Kidney
Integrins
In Vitro Techniques
Organ Culture Techniques
Aspartic Acid
Glycine
Arginine
Anti-Idiotypic Antibodies
Apoptosis
Staining and Labeling
Messenger RNA
Peptides
Antibodies
Wounds and Injuries

Keywords

  • Arginine-glycine-aspartie acid
  • Metanephric blastema
  • Renal organogenesis
  • Ureteric bud
  • α(v)β-integrin

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Rogers, S. A., Padanilam, B. J., Hruska, K. A., Giachelli, C. M., & Hammerman, M. R. (1997). Metanephric osteopontin regulates nephrogenesis in vitro. American Journal of Physiology - Renal Physiology, 272(4 41-4), F469-F476.

Metanephric osteopontin regulates nephrogenesis in vitro. / Rogers, Sharon A.; Padanilam, Babu J.; Hruska, Keith A.; Giachelli, Cecilia M.; Hammerman, Marc R.

In: American Journal of Physiology - Renal Physiology, Vol. 272, No. 4 41-4, 01.04.1997, p. F469-F476.

Research output: Contribution to journalArticle

Rogers, SA, Padanilam, BJ, Hruska, KA, Giachelli, CM & Hammerman, MR 1997, 'Metanephric osteopontin regulates nephrogenesis in vitro', American Journal of Physiology - Renal Physiology, vol. 272, no. 4 41-4, pp. F469-F476.
Rogers SA, Padanilam BJ, Hruska KA, Giachelli CM, Hammerman MR. Metanephric osteopontin regulates nephrogenesis in vitro. American Journal of Physiology - Renal Physiology. 1997 Apr 1;272(4 41-4):F469-F476.
Rogers, Sharon A. ; Padanilam, Babu J. ; Hruska, Keith A. ; Giachelli, Cecilia M. ; Hammerman, Marc R. / Metanephric osteopontin regulates nephrogenesis in vitro. In: American Journal of Physiology - Renal Physiology. 1997 ; Vol. 272, No. 4 41-4. pp. F469-F476.
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