Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula

Yukari Maezato, Tyler Johnson, Samuel McCarthy, Karl Dana, Paul H Blum

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Archaea such as Metallosphaera sedula are thermophilic lithoautotrophs that occupy unusually acidic and metal-rich environments. These traits are thought to underlie their industrial importance for bioleaching of base and precious metals. In this study, a genetic approach was taken to investigate the specific relationship between metal resistance and lithoautotrophy during biotransformation of the primary copper ore, chalcopyrite (CuFeS2). In this study, a genetic system was developed for M. sedula to investigate parameters that limit bioleaching of chalcopyrite. The functionalrole of the M. sedula copRTA operon was demonstrated by cross-species complementation of a copper-sensitive Sulfolobus solfataricus copR mutant. Inactivation of the gene encoding the M. sedula copper efflux protein, copA, using targeted recombination compromised metal resistance and eliminated chalcopyrite bioleaching. In contrast, a spontaneous M. sedula mutant (CuR1) with elevated metal resistance transformed chalcopyrite at an accelerated rate without affecting chemoheterotrophic growth. Proteomic analysis of CuR1 identified pleiotropic changes, including altered abundance of transport proteins having AAA-ATPase motifs. Addition of the insoluble carbonate mineral witherite (BaCO3) further stimulated chalcopyrite lithotrophy, indicating that carbon was a limiting factor. Since both mineral types were actively colonized, enhanced metal leaching may arise from the cooperative exchange of energy and carbon between surface-adhered populations. Genetic approaches provide a new means of improving the efficiency of metal bioleaching by enhancing the mechanistic understanding of thermophilic lithoautotrophy.

Original languageEnglish (US)
Pages (from-to)6856-6863
Number of pages8
JournalJournal of bacteriology
Volume194
Issue number24
DOIs
StatePublished - Oct 1 2012

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Sulfolobaceae
Metals
Copper
Minerals
Carbon
Autotrophic Processes
Sulfolobus solfataricus
Carbonates
Archaea
Gene Silencing
Operon
Biotransformation
Proteomics
Genetic Recombination
Adenosine Triphosphatases
Carrier Proteins
chalcopyrite

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula. / Maezato, Yukari; Johnson, Tyler; McCarthy, Samuel; Dana, Karl; Blum, Paul H.

In: Journal of bacteriology, Vol. 194, No. 24, 01.10.2012, p. 6856-6863.

Research output: Contribution to journalArticle

Maezato, Yukari ; Johnson, Tyler ; McCarthy, Samuel ; Dana, Karl ; Blum, Paul H. / Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula. In: Journal of bacteriology. 2012 ; Vol. 194, No. 24. pp. 6856-6863.
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