Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA

Scott E. Andersen, Dhundy R. Bastola, Subhash C. Minocha

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The metabolisms of arginine (Arg), ornithine (Orn), and putrescine were compared in a nontransgenic and a transgenic cell line of carrot (Daucus carota L.) expressing a mouse Orn decarboxylase cDNA. [14C]Arg, [14C]Orn, and [14C]putrescine were fed to cells and their rates of decarboxylation, uptake, metabolism into polyamines, and incorporation into acid-insoluble material were determined. Transgenic cells showed higher decarboxylation rates for labeled Orn than the nontransgenic cells. This was correlated positively with higher amounts of labeled putrescine production from labeled Orn. With labeled Arg, both the transgenic and the nontransgenic cells exhibited similar rates of decarboxylation and conversion into labeled putrescine. When [14C]putrescine was fed, higher rates of degradation were observed in transgenic cells as compared with the nontransgenic cells. It is concluded that (a) increased production of putrescine via the Orn decarboxylase pathway has no compensatory effects on the Arg decarboxylase pathway, and (b) higher rates of putrescine production in the transgenic cells are accompanied by higher rates of putrescine conversion into spermidine and spermine as well as the catabolism of putrescine.

Original languageEnglish (US)
Pages (from-to)299-307
Number of pages9
JournalPlant physiology
Volume116
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

ornithine decarboxylase
Daucus carota
Ornithine Decarboxylase
Putrescine
Polyamines
putrescine
polyamines
carrots
Complementary DNA
genetically modified organisms
metabolism
mice
Ornithine
ornithine
Decarboxylation
decarboxylation
cells
arginine
Arginine
arginine decarboxylase

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA. / Andersen, Scott E.; Bastola, Dhundy R.; Minocha, Subhash C.

In: Plant physiology, Vol. 116, No. 1, 01.01.1998, p. 299-307.

Research output: Contribution to journalArticle

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