Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content

Edgar B Cahoon, Sarah E. Hall, Kevin G. Ripp, Thaya S. Ganzke, William D. Hitz, Sean J. Coughlan

Research output: Contribution to journalArticle

253 Citations (Scopus)

Abstract

Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catalyzes the committed step of tocotrienol biosynthesis, were isolated from barley, wheat and rice seeds. Transgenic expression of the barley HGGT in Arabidopsis thaliana leaves resulted in accumulation of tocotrienols, which were absent from leaves of nontransformed plants, and a 10- to 15-fold increase in total vitamin E antioxidants (tocotrienols plus tocopherols). Overexpression of the barley HGGT in corn seeds resulted in an increase in tocotrienol and tocopherol content of as much as six-fold. These results provide insight into the genetic basis for tocotrienol biosynthesis in plants and demonstrate the ability to enhance the antioxidant content of crops by introduction of an enzyme that redirects metabolic flux.

Original languageEnglish (US)
Pages (from-to)1082-1087
Number of pages6
JournalNature Biotechnology
Volume21
Issue number9
DOIs
StatePublished - Sep 1 2003

Fingerprint

Tocotrienols
Vitamins
Biosynthesis
Antioxidants
Vitamin E
Seed
Homogentisic Acid
Acids
Hordeum
Transferases
Seeds
Nutrition
Tocopherols
Farms
Crops
Triticum
Enzymes
Fluxes
Plant Leaves
Nutritive Value

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. / Cahoon, Edgar B; Hall, Sarah E.; Ripp, Kevin G.; Ganzke, Thaya S.; Hitz, William D.; Coughlan, Sean J.

In: Nature Biotechnology, Vol. 21, No. 9, 01.09.2003, p. 1082-1087.

Research output: Contribution to journalArticle

Cahoon, Edgar B ; Hall, Sarah E. ; Ripp, Kevin G. ; Ganzke, Thaya S. ; Hitz, William D. ; Coughlan, Sean J. / Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. In: Nature Biotechnology. 2003 ; Vol. 21, No. 9. pp. 1082-1087.
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