Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm

Anji Reddy Konda, Tara J. Nazarenus, Hanh Nguyen, Junsi Yang, Malleswari Gelli, Samantha Swenson, Jamie M. Shipp, Monica A. Schmidt, Rebecca E. Cahoon, Ozan N. Ciftci, Chunyu Zhang, Tom Elmo Clemente, Edgar B. Cahoon

Research output: Contribution to journalArticle

Abstract

Soybean seeds produce oil enriched in oxidatively unstable polyunsaturated fatty acids (PUFAs) and are also a potential biotechnological platform for synthesis of oils with nutritional omega-3 PUFAs. In this study, we engineered soybeans for seed-specific expression of a barley homogentisate geranylgeranyl transferase (HGGT) transgene alone and with a soybean γ-tocopherol methyltransferase (γ-TMT) transgene. Seeds for HGGT-expressing lines had 8- to 10-fold increases in total vitamin E tocochromanols, principally as tocotrienols, with little effect on seed oil or protein concentrations. Tocochromanols were primarily in δ- and γ-forms, which were shifted largely to α- and β-tocochromanols with γ-TMT co-expression. We tested whether oxidative stability of conventional or PUFA-enhanced soybean oil could be improved by metabolic engineering for increased vitamin E antioxidants. Selected lines were crossed with a stearidonic acid (SDA, 18:4Δ6,9,12,15)-producing line, resulting in progeny with oil enriched in SDA and α- or γ-linoleic acid (ALA, 18:3Δ9,12,15 or GLA, 18:3Δ6,9,12), from transgene segregation. Oil extracted from HGGT-expressing lines had ≥6-fold increase in free radical scavenging activity compared to controls. However, the oxidative stability index of oil from vitamin E-enhanced lines was ~15% lower than that of oil from non-engineered seeds and nearly the same or modestly increased in oil from the GLA, ALA and SDA backgrounds relative to controls. These findings show that soybean is an effective platform for producing high levels of free-radical scavenging vitamin E antioxidants, but this trait may have negative effects on oxidative stability of conventional oil or only modest improvement of the oxidative stability of PUFA-enhanced oil.

Original languageEnglish (US)
Pages (from-to)63-73
Number of pages11
JournalMetabolic Engineering
Volume57
DOIs
StatePublished - Jan 2020

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Polyunsaturated fatty acids
Metabolic engineering
Metabolic Engineering
Vitamins
Antioxidants
Unsaturated Fatty Acids
Vitamin E
Soybeans
Seed
Seeds
Oils
Transferases
Oilseeds
Transgenes
Scavenging
Free radicals
Free Radicals
Tocotrienols
Linoleic acid
Soybean oil

Keywords

  • Antioxidant
  • Homogentisate geranylgeranyl transferase
  • Oilseed
  • Soybean
  • Tocochromanol
  • Tocopherol
  • Tocotrienol
  • Vitamin E

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm. / Konda, Anji Reddy; Nazarenus, Tara J.; Nguyen, Hanh; Yang, Junsi; Gelli, Malleswari; Swenson, Samantha; Shipp, Jamie M.; Schmidt, Monica A.; Cahoon, Rebecca E.; Ciftci, Ozan N.; Zhang, Chunyu; Clemente, Tom Elmo; Cahoon, Edgar B.

In: Metabolic Engineering, Vol. 57, 01.2020, p. 63-73.

Research output: Contribution to journalArticle

Konda, AR, Nazarenus, TJ, Nguyen, H, Yang, J, Gelli, M, Swenson, S, Shipp, JM, Schmidt, MA, Cahoon, RE, Ciftci, ON, Zhang, C, Clemente, TE & Cahoon, EB 2020, 'Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm', Metabolic Engineering, vol. 57, pp. 63-73. https://doi.org/10.1016/j.ymben.2019.10.005
Konda, Anji Reddy ; Nazarenus, Tara J. ; Nguyen, Hanh ; Yang, Junsi ; Gelli, Malleswari ; Swenson, Samantha ; Shipp, Jamie M. ; Schmidt, Monica A. ; Cahoon, Rebecca E. ; Ciftci, Ozan N. ; Zhang, Chunyu ; Clemente, Tom Elmo ; Cahoon, Edgar B. / Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm. In: Metabolic Engineering. 2020 ; Vol. 57. pp. 63-73.
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AU - Yang, Junsi

AU - Gelli, Malleswari

AU - Swenson, Samantha

AU - Shipp, Jamie M.

AU - Schmidt, Monica A.

AU - Cahoon, Rebecca E.

AU - Ciftci, Ozan N.

AU - Zhang, Chunyu

AU - Clemente, Tom Elmo

AU - Cahoon, Edgar B.

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N2 - Soybean seeds produce oil enriched in oxidatively unstable polyunsaturated fatty acids (PUFAs) and are also a potential biotechnological platform for synthesis of oils with nutritional omega-3 PUFAs. In this study, we engineered soybeans for seed-specific expression of a barley homogentisate geranylgeranyl transferase (HGGT) transgene alone and with a soybean γ-tocopherol methyltransferase (γ-TMT) transgene. Seeds for HGGT-expressing lines had 8- to 10-fold increases in total vitamin E tocochromanols, principally as tocotrienols, with little effect on seed oil or protein concentrations. Tocochromanols were primarily in δ- and γ-forms, which were shifted largely to α- and β-tocochromanols with γ-TMT co-expression. We tested whether oxidative stability of conventional or PUFA-enhanced soybean oil could be improved by metabolic engineering for increased vitamin E antioxidants. Selected lines were crossed with a stearidonic acid (SDA, 18:4Δ6,9,12,15)-producing line, resulting in progeny with oil enriched in SDA and α- or γ-linoleic acid (ALA, 18:3Δ9,12,15 or GLA, 18:3Δ6,9,12), from transgene segregation. Oil extracted from HGGT-expressing lines had ≥6-fold increase in free radical scavenging activity compared to controls. However, the oxidative stability index of oil from vitamin E-enhanced lines was ~15% lower than that of oil from non-engineered seeds and nearly the same or modestly increased in oil from the GLA, ALA and SDA backgrounds relative to controls. These findings show that soybean is an effective platform for producing high levels of free-radical scavenging vitamin E antioxidants, but this trait may have negative effects on oxidative stability of conventional oil or only modest improvement of the oxidative stability of PUFA-enhanced oil.

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