Metabolic constraints on magnaporthe biotrophy: Loss of de novo asparagine biosynthesis aborts invasive Hyphal growth in the first infected rice cell

Margarita Marroquin-Guzman, Juliana Krotz, Harriet Appeah, Richard A. Wilson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The blast fungus Magnaporthe oryzae devastates global rice yields and is an emerging threat to wheat. Determining the metabolic strategies underlying M. oryzae growth in host cells could lead to the development of new plant protection approaches against blast. Here, we targeted asparagine synthetase (encoded by ASN1), which is required for the terminal step in asparagine production from aspartate and glutamine, the sole pathway to de novo asparagine biosynthesis in M. oryzae. Consequently, the Δasn1 mutant strains could not grow on minimal media without asparagine supplementation. Spores harvested from supplemented plates could form appressoria and penetrate rice leaf surfaces, but biotrophic growth was aborted and the Δasn1 strains were nonpathogenic. This work provides strong genetic evidence that de novo asparagines biosynthesis, and not acquisition from the host, is a critical and potentially exploitable metabolic strategy employed by M. oryzae in order to successfully colonize rice cells.

Original languageEnglish (US)
Article number000713
Pages (from-to)1541-1546
Number of pages6
JournalMicrobiology (United Kingdom)
Volume164
Issue number12
DOIs
StatePublished - Jan 1 2018

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Magnaporthe
Asparagine
Growth
Aspartate-Ammonia Ligase
Plant Development
Oryza
Spores
Glutamine
Aspartic Acid
Triticum
Fungi

ASJC Scopus subject areas

  • Microbiology

Cite this

Metabolic constraints on magnaporthe biotrophy : Loss of de novo asparagine biosynthesis aborts invasive Hyphal growth in the first infected rice cell. / Marroquin-Guzman, Margarita; Krotz, Juliana; Appeah, Harriet; Wilson, Richard A.

In: Microbiology (United Kingdom), Vol. 164, No. 12, 000713, 01.01.2018, p. 1541-1546.

Research output: Contribution to journalArticle

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