Metabolic characterization of a Sirt5 deficient mouse model

Jiujiu Yu, Sushabhan Sadhukhan, Lilia G. Noriega, Norman Moullan, Bin He, Robert S. Weiss, Hening Lin, Kristina Schoonjans, Johan Auwerx

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Sirt5, localized in the mitochondria, is a member of sirtuin family of NAD +dependent deacetylases. Sirt5 was shown to deacetylate and activate carbamoyl phosphate synthase 1. Most recently, Sirt5 was reported to be the predominant protein desuccinylase and demalonylase in the mitochondria because the ablation of Sirt5 enhanced the global succinylation and malonylation of mitochondrial proteins, including many metabolic enzymes. In order to determine the physiological role of Sirt5 in metabolic homeostasis, we generated a germline Sirt5 deficient (Sirt5-/-) mouse model and performed a thorough metabolic characterization of this mouse line. Although a global protein hypersuccinylation and elevated serum ammonia during fasting were observed in our Sirt5-/-mouse model, Sirt5 deficiency did not lead to any overt metabolic abnormalities under either chow or high fat diet conditions. These observations suggest that Sirt5 is likely to be dispensable for the metabolic homeostasis under the basal conditions.

Original languageEnglish (US)
Article number2806
JournalScientific reports
Volume3
DOIs
StatePublished - Oct 14 2013

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Mitochondria
Homeostasis
Carbamyl Phosphate
Mitochondrial Proteins
High Fat Diet
Ammonia
NAD
Fasting
Proteins
Enzymes
Serum

ASJC Scopus subject areas

  • General

Cite this

Yu, J., Sadhukhan, S., Noriega, L. G., Moullan, N., He, B., Weiss, R. S., ... Auwerx, J. (2013). Metabolic characterization of a Sirt5 deficient mouse model. Scientific reports, 3, [2806]. https://doi.org/10.1038/srep02806

Metabolic characterization of a Sirt5 deficient mouse model. / Yu, Jiujiu; Sadhukhan, Sushabhan; Noriega, Lilia G.; Moullan, Norman; He, Bin; Weiss, Robert S.; Lin, Hening; Schoonjans, Kristina; Auwerx, Johan.

In: Scientific reports, Vol. 3, 2806, 14.10.2013.

Research output: Contribution to journalArticle

Yu, J, Sadhukhan, S, Noriega, LG, Moullan, N, He, B, Weiss, RS, Lin, H, Schoonjans, K & Auwerx, J 2013, 'Metabolic characterization of a Sirt5 deficient mouse model', Scientific reports, vol. 3, 2806. https://doi.org/10.1038/srep02806
Yu J, Sadhukhan S, Noriega LG, Moullan N, He B, Weiss RS et al. Metabolic characterization of a Sirt5 deficient mouse model. Scientific reports. 2013 Oct 14;3. 2806. https://doi.org/10.1038/srep02806
Yu, Jiujiu ; Sadhukhan, Sushabhan ; Noriega, Lilia G. ; Moullan, Norman ; He, Bin ; Weiss, Robert S. ; Lin, Hening ; Schoonjans, Kristina ; Auwerx, Johan. / Metabolic characterization of a Sirt5 deficient mouse model. In: Scientific reports. 2013 ; Vol. 3.
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