Met receptor inhibitor SU11274 localizes in the endoplasmic reticulum

Edwin J. Wiest, Heather Jensen Smith, Michael A Hollingsworth

Research output: Contribution to journalArticle

Abstract

We discovered that SU11274, a class I c-Met inhibitor, fluoresces when excited by 488 nm laser light and showed rapid specific accumulation in distinct subcellular compartments. Given that SU11274 reduces cancer cell viability, we exploited these newly identified spectral properties to determine SU11274 intracellular distribution and accumulation in human pancreatic cancer cells. The aim of the studies reported here was to identify organelle(s) to which SU11274 is trafficked. We conclude that SU11274 rapidly and predominantly accumulates in the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)858-862
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume501
Issue number4
DOIs
StatePublished - Jul 2 2018

Fingerprint

Endoplasmic Reticulum
Cells
Pancreatic Neoplasms
Organelles
Cell Survival
Lasers
((3Z)-N-(3-chlorophenyl)-3-((3,5-dimethyl-4-((4-methylpiperazin-1-yl)carbonyl)-1H-pyrrol-2-yl)methylene)-N-methyl-2-oxo-2,3-dihydro-1H-indole-5-sulfonamide)
Light
Neoplasms

Keywords

  • Endoplasmic reticulum
  • SU11274
  • Sulfonylurea receptor
  • Unfolded protein response

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Met receptor inhibitor SU11274 localizes in the endoplasmic reticulum. / Wiest, Edwin J.; Smith, Heather Jensen; Hollingsworth, Michael A.

In: Biochemical and Biophysical Research Communications, Vol. 501, No. 4, 02.07.2018, p. 858-862.

Research output: Contribution to journalArticle

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