Membrane lipid biosynthesis in the philly mouse Lens. I. The major phospholipid classes

J. Stevens Andrews, Tom Leonard-Martin, Peter F Kador

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The presence of toluidine blue-staining vesicles and leaky membranes in the Philly mouse lens as early as 10 days after birth led us to investigate the phospholipid metabolism of these lenses. On a dry weight, or per lens, basis, the Philly mouse lens had a slightly higher than normal concentration of phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), sphingomyelin (S) and cholesterol (C) at 10 days. The levels of PC and PE dropped between 10 and 30 days in the Philly mouse, while in the control lenses the amounts of both phospholipids, on a per lens basis, was increasing. P incorporation indicated an increased rate of turnover in the Philly lenses. Cholesterol and sphingomyelin concentrations closely paralleled the dry weight per lens curves in the Philly and control, Swiss-Webster, lenses. Sphingonyelin specific activity dropped rapidly in the first 10 day period, possibly reflecting the lowered PC pool available for S biosynthesis. These results suggest a lower utilization of biosynthesized lipids for membrane assembly in the Philly lens. The observed higher turnover of metabolically-active phospholipid supports the presence of increased degradative activity which may, in turn, result in leaky membranes and, thus, contribute to cataract development.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalCurrent Eye Research
Volume3
Issue number2
DOIs
StatePublished - Jan 1 1984

Fingerprint

Membrane Lipids
Lenses
Phospholipids
Phosphatidylcholines
Ethanolamine
Sphingomyelins
Cholesterol
Weights and Measures
Tolonium Chloride
Membranes
Cataract
Parturition
Staining and Labeling

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Membrane lipid biosynthesis in the philly mouse Lens. I. The major phospholipid classes. / Andrews, J. Stevens; Leonard-Martin, Tom; Kador, Peter F.

In: Current Eye Research, Vol. 3, No. 2, 01.01.1984, p. 279-285.

Research output: Contribution to journalArticle

Andrews, J. Stevens ; Leonard-Martin, Tom ; Kador, Peter F. / Membrane lipid biosynthesis in the philly mouse Lens. I. The major phospholipid classes. In: Current Eye Research. 1984 ; Vol. 3, No. 2. pp. 279-285.
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