Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus

Hiroshi Hamana, Hideaki Moriyama, Takao Shinozawa, Nobuo Tanaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Crystallization of orotate phosphoribosyltransferase from a thermophilic organism, Thermus thermophilus, was achieved using the hanging-drop vapour-diffusion method coupled with a macroseeding starter. Small needle-like microcrystals were grown in a fresh protein solution in the presence of 2-methyl-2,4-pentanediol at 298 K or below. Although these normal temperature conditions caused stacking crystallization, an increase of temperature to 310K permitted crystal growth. This was because of increased enzyme solubility at the higher temperature. The crystal was found to belong to the monoclinic space group P21 with unit-cell parameters a = 44.4, b = 59.6, c = 67.8 Å and β = 98.3°.

Original languageEnglish (US)
Pages (from-to)345-346
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number1
DOIs
StatePublished - Jan 1 1999

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Orotate Phosphoribosyltransferase
Thermus thermophilus
Crystallization
starters
Single crystals
crystallization
Temperature
single crystals
microcrystals
organisms
needles
enzymes
crystal growth
Microcrystals
Starters
solubility
vapors
proteins
Needles
Solubility

ASJC Scopus subject areas

  • Structural Biology

Cite this

Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus. / Hamana, Hiroshi; Moriyama, Hideaki; Shinozawa, Takao; Tanaka, Nobuo.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 1, 01.01.1999, p. 345-346.

Research output: Contribution to journalArticle

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