Medium-dependent antibacterial properties and bacterial filtration ability of reduced graphene oxide

Alexander Gusev, Olga Zakharova, Dmitry S. Muratov, Nataliia S. Vorobeva, Mamun Sarker, Iaroslav Rybkin, Daniil Bratashov, Evgeny Kolesnikov, Aleš Lapanje, Denis V. Kuznetsov, Alexander Sinitskii

Research output: Contribution to journalArticle

Abstract

Toxicity of reduced graphene oxide (rGO) has been a topic of multiple studies and was shown to depend on a variety of characteristics of rGO and biological objects of interest. In this paper, we demonstrate that when studying the same dispersions of rGO and fluorescent Escherichia coli (E. coli) bacteria, the outcome of nanotoxicity experiments also depends on the type of culture medium. We show that rGO inhibits the growth of bacteria in a nutrition medium but shows little effect on the behavior of E. coli in a physiological saline solution. The observed effects of rGO on E. coli in different media could be at least partially rationalized through the adsorption of bacteria and nutrients on the dispersed rGO sheets, which is likely mediated via hydrogen bonding. We also found that the interaction between rGO and E. coli is medium-dependent, and in physiological saline solutions they form stable flocculate structures that were not observed in nutrition media. Furthermore, the aggregation of rGO and E. coli in saline media was observed regardless of whether the bacteria were alive or dead. Filtration of the aggregate suspensions led to nearly complete removal of bacteria from filtered liquids, which highlights the potential of rGO for the filtration and separation of biological contaminants, regardless of whether they include live or dead microorganisms.

Original languageEnglish (US)
Article number1454
JournalNanomaterials
Volume9
Issue number10
DOIs
StatePublished - Oct 2019

Fingerprint

Graphite
Oxides
Graphene
Escherichia coli
Bacteria
Nutrition
Sodium Chloride
Dispersions
Microorganisms
Nutrients
Toxicity
Culture Media
Suspensions
Hydrogen bonds
Agglomeration
Impurities
Adsorption
Liquids

Keywords

  • Antibacterial properties
  • Escherichia coli
  • Graphene oxide
  • Nanotoxicity

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

Cite this

Medium-dependent antibacterial properties and bacterial filtration ability of reduced graphene oxide. / Gusev, Alexander; Zakharova, Olga; Muratov, Dmitry S.; Vorobeva, Nataliia S.; Sarker, Mamun; Rybkin, Iaroslav; Bratashov, Daniil; Kolesnikov, Evgeny; Lapanje, Aleš; Kuznetsov, Denis V.; Sinitskii, Alexander.

In: Nanomaterials, Vol. 9, No. 10, 1454, 10.2019.

Research output: Contribution to journalArticle

Gusev, A, Zakharova, O, Muratov, DS, Vorobeva, NS, Sarker, M, Rybkin, I, Bratashov, D, Kolesnikov, E, Lapanje, A, Kuznetsov, DV & Sinitskii, A 2019, 'Medium-dependent antibacterial properties and bacterial filtration ability of reduced graphene oxide', Nanomaterials, vol. 9, no. 10, 1454. https://doi.org/10.3390/nano9101454
Gusev A, Zakharova O, Muratov DS, Vorobeva NS, Sarker M, Rybkin I et al. Medium-dependent antibacterial properties and bacterial filtration ability of reduced graphene oxide. Nanomaterials. 2019 Oct;9(10). 1454. https://doi.org/10.3390/nano9101454
Gusev, Alexander ; Zakharova, Olga ; Muratov, Dmitry S. ; Vorobeva, Nataliia S. ; Sarker, Mamun ; Rybkin, Iaroslav ; Bratashov, Daniil ; Kolesnikov, Evgeny ; Lapanje, Aleš ; Kuznetsov, Denis V. ; Sinitskii, Alexander. / Medium-dependent antibacterial properties and bacterial filtration ability of reduced graphene oxide. In: Nanomaterials. 2019 ; Vol. 9, No. 10.
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