Mechanism of extracellular domain-deleted dominant negative cadherins

Marvin T. Nieman, Jae Beom Kim, Keith R Johnson, Margaret J. Wheelock

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The cadherin/catenin complex mediates Ca2+-dependent cell-cell interactions that are essential for normal developmental processes. It has been proposed that sorting of cells during embryonic development is due, at least in part, to expression of different cadherin family members or to expression of differing levels of a single family member. Expression of dominant-negative cadherins has been used experimentally to decrease cell-cell interactions in whole organisms and in cultured cells. In this study, we elucidated the mechanism of action of extracellular domain-deleted dominant-negative cadherin, showing that it is not cadherin isotype-specific, and that it must be membrane-associated but the orientation within the membrane does not matter. In addition, membrane-targeted cytoplasmic domain cadherin with tile catenin-binding domain deleted does not function as a dominant-negative cadherin. Expression of extracellular domain-deleted dominant-negative cadherin results in down-regulation of endogenous cadherins which presumably contributes to the non-adhesive phenotype.

Original languageEnglish (US)
Pages (from-to)1621-1632
Number of pages12
JournalJournal of Cell Science
Volume112
Issue number10
StatePublished - Jun 16 1999

Fingerprint

Cadherins
Catenins
Cell Communication
Membranes
Embryonic Development
Cultured Cells
Down-Regulation
Cell Membrane
Phenotype

Keywords

  • Adherens junction
  • Catenin
  • Cell adhesion
  • E-cadherin
  • N-cadherin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nieman, M. T., Kim, J. B., Johnson, K. R., & Wheelock, M. J. (1999). Mechanism of extracellular domain-deleted dominant negative cadherins. Journal of Cell Science, 112(10), 1621-1632.

Mechanism of extracellular domain-deleted dominant negative cadherins. / Nieman, Marvin T.; Kim, Jae Beom; Johnson, Keith R; Wheelock, Margaret J.

In: Journal of Cell Science, Vol. 112, No. 10, 16.06.1999, p. 1621-1632.

Research output: Contribution to journalArticle

Nieman, MT, Kim, JB, Johnson, KR & Wheelock, MJ 1999, 'Mechanism of extracellular domain-deleted dominant negative cadherins', Journal of Cell Science, vol. 112, no. 10, pp. 1621-1632.
Nieman, Marvin T. ; Kim, Jae Beom ; Johnson, Keith R ; Wheelock, Margaret J. / Mechanism of extracellular domain-deleted dominant negative cadherins. In: Journal of Cell Science. 1999 ; Vol. 112, No. 10. pp. 1621-1632.
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