Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter

Tahir H. Tahirov, Ko Sato, Emi Ichikawa-Iwata, Motoko Sasaki, Taiko Inoue-Bungo, Masaaki Shiina, Kazumi Kimura, Shioka Takata, Atsushi Fujikawa, Hisayuki Morii, Takashi Kumasaka, Masaki Yamamoto, Shunsuke Ishii, Kazuhiro Ogata

Research output: Contribution to journalArticle

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Abstract

c-Myb, but not avian myeloblastosis virus (AMV) v-Myb, cooperates with C/EBPβ to regulate transcription of myeloid-specific genes. To assess the structural basis for that difference, we determined the crystal structures of complexes comprised of the c-Myb or AMV v-Myb DNA-binding domain (DBD), the C/EBPβ DBD, and a promoter DNA fragment. Within the c-Myb complex, a DNA-bound C/EBPβ interacts with R2 of c-Myb bound to a different DNA fragment; point mutations in v-Myb R2 eliminate such interaction within the v-Myb complex. GST pull-down assays, luciferase trans-activation assays, and atomic force microscopy confirmed that the interaction of c-Myb and C/EBPβ observed in crystal mimics their long range interaction on the promoter, which is accompanied by intervening DNA looping.

Original languageEnglish (US)
Pages (from-to)57-70
Number of pages14
JournalCell
Volume108
Issue number1
DOIs
StatePublished - Jan 11 2002

Fingerprint

DNA
Avian Myeloblastosis Virus
Viruses
Assays
Atomic Force Microscopy
Transcription
Luciferases
Point Mutation
Atomic force microscopy
Genes
Crystal structure
Chemical activation
Crystals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tahirov, T. H., Sato, K., Ichikawa-Iwata, E., Sasaki, M., Inoue-Bungo, T., Shiina, M., ... Ogata, K. (2002). Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter. Cell, 108(1), 57-70. https://doi.org/10.1016/S0092-8674(01)00636-5

Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter. / Tahirov, Tahir H.; Sato, Ko; Ichikawa-Iwata, Emi; Sasaki, Motoko; Inoue-Bungo, Taiko; Shiina, Masaaki; Kimura, Kazumi; Takata, Shioka; Fujikawa, Atsushi; Morii, Hisayuki; Kumasaka, Takashi; Yamamoto, Masaki; Ishii, Shunsuke; Ogata, Kazuhiro.

In: Cell, Vol. 108, No. 1, 11.01.2002, p. 57-70.

Research output: Contribution to journalArticle

Tahirov, TH, Sato, K, Ichikawa-Iwata, E, Sasaki, M, Inoue-Bungo, T, Shiina, M, Kimura, K, Takata, S, Fujikawa, A, Morii, H, Kumasaka, T, Yamamoto, M, Ishii, S & Ogata, K 2002, 'Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter', Cell, vol. 108, no. 1, pp. 57-70. https://doi.org/10.1016/S0092-8674(01)00636-5
Tahirov TH, Sato K, Ichikawa-Iwata E, Sasaki M, Inoue-Bungo T, Shiina M et al. Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter. Cell. 2002 Jan 11;108(1):57-70. https://doi.org/10.1016/S0092-8674(01)00636-5
Tahirov, Tahir H. ; Sato, Ko ; Ichikawa-Iwata, Emi ; Sasaki, Motoko ; Inoue-Bungo, Taiko ; Shiina, Masaaki ; Kimura, Kazumi ; Takata, Shioka ; Fujikawa, Atsushi ; Morii, Hisayuki ; Kumasaka, Takashi ; Yamamoto, Masaki ; Ishii, Shunsuke ; Ogata, Kazuhiro. / Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter. In: Cell. 2002 ; Vol. 108, No. 1. pp. 57-70.
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