Mechanical stretch induces angiotensinogen expression through PARP1 activation in kidney proximal tubular cells

Jeong Soon Lee, Jung Y Lim, Jinu Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Poly(ADP-ribose) polymerase 1 (PARP1) contributes to fibrosis in several disease models. Recent in vivo data indicate that loss of PARP1 attenuates renal fibrosis and inflammation independent on transforming growth factor-β (TGF-β); however, the role of PARP1 in kidney tubular cells in response to tubulointerstitial fibrosis remains to be defined. Here, we report that PARP1 activation after mechanical stretch of kidney proximal tubular cells enhances angiotensinogen expression via nuclear factor kappa B (NF-κB) activation. Mechanical stretch for 24 h increased PARP1 expression and activation in mouse cortical proximal tubular (MCT) cells. Treatment with 3-aminobenzamide, a PARP1 inhibitor, efficaciously reduced the PARP1 activation induced by mechanical stretch. PARP1 inhibition also reduced angiotensinogen expression and NF-κB p65 phosphorylation induced by mechanical stretch. TGF-β1 expression and secretion were enhanced by mechanical stretch, but PARP1 inhibition did not change the levels of TGF-β1. These data demonstrate that mechanical stretch-induced PARP1 activation contributes to angiotensinogen expression and NF-κB activation in kidney proximal tubular cells, resulting in the promotion of renal tubulointerstitial fibrosis and inflammation.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume51
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Angiotensinogen
Kidney
NF-kappa B
Transforming Growth Factors
Fibrosis
Poly (ADP-Ribose) Polymerase-1
Inflammation
Phosphorylation

Keywords

  • Angiotensinogen
  • Kidney proximal tubular cells
  • Mechanical stretch
  • NF-κB
  • Poly(ADP-ribose) polymerase 1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Mechanical stretch induces angiotensinogen expression through PARP1 activation in kidney proximal tubular cells. / Lee, Jeong Soon; Lim, Jung Y; Kim, Jinu.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 51, No. 1, 01.01.2014, p. 72-78.

Research output: Contribution to journalArticle

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