Mechanical loading activates β-catenin signaling in periodontal ligament cells

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective: To determine whether β-catenin signaling is responsive to mechanical loading in periodontal ligament (PDL) cells. Materials and Methods: To determine whether Wnt/β-catenin signaling pathway components are present and functional, PDL cells were treated with lithium chloride or Wnt3a-conditioned media. To determine whether mechanical strain activates β-catenin signaling, PDL cells were subjected to compressive loading. Activation of the β-catenin signaling pathway was determined by immunofluorescence, Western immunoblotting, and TOPflash assay. Results: Mimicking Wnt signaling stimulates β-catenin nuclear translocation and T-cell factor/ lymphoid enhancer binding factor-dependent transcriptional activation in PDL cells. Mechanical loading stimulates a transient accumulation of dephosphorylated β-catenin in the cytoplasm and its translocation to the nucleus. This effect of strain acts through activation of protein kinase B and phosphorylation of glycogen synthase kinase-3 beta. These strain-related changes do not involve the low-density lipoprotein receptor-related protein 5/Wnt receptor. Conclusions: The Wnt/β-catenin signaling pathway components are functional and activated by mechanical loading in PDL cells. β-catenin serves as an effector of mechanical signals in PDL cells.

Original languageEnglish (US)
Pages (from-to)592-599
Number of pages8
JournalAngle Orthodontist
Volume81
Issue number4
DOIs
StatePublished - Jul 1 2011

Fingerprint

Catenins
Periodontal Ligament
TCF Transcription Factors
Wnt Signaling Pathway
Low Density Lipoprotein Receptor-Related Protein-5
Wnt Receptors
Lithium Chloride
Proto-Oncogene Proteins c-akt
Conditioned Culture Medium
Transcriptional Activation
Fluorescent Antibody Technique
Cytoplasm
Western Blotting
Phosphorylation

Keywords

  • Mechanical loading
  • Periodontal ligament cells
  • Tooth movement
  • β-catenin

ASJC Scopus subject areas

  • Orthodontics

Cite this

Mechanical loading activates β-catenin signaling in periodontal ligament cells. / Premaraj, Sundaralingam; Souza, Isabela; Premaraj, Thyagaseely.

In: Angle Orthodontist, Vol. 81, No. 4, 01.07.2011, p. 592-599.

Research output: Contribution to journalArticle

@article{d3d7045fcb444385be3fcb4373839655,
title = "Mechanical loading activates β-catenin signaling in periodontal ligament cells",
abstract = "Objective: To determine whether β-catenin signaling is responsive to mechanical loading in periodontal ligament (PDL) cells. Materials and Methods: To determine whether Wnt/β-catenin signaling pathway components are present and functional, PDL cells were treated with lithium chloride or Wnt3a-conditioned media. To determine whether mechanical strain activates β-catenin signaling, PDL cells were subjected to compressive loading. Activation of the β-catenin signaling pathway was determined by immunofluorescence, Western immunoblotting, and TOPflash assay. Results: Mimicking Wnt signaling stimulates β-catenin nuclear translocation and T-cell factor/ lymphoid enhancer binding factor-dependent transcriptional activation in PDL cells. Mechanical loading stimulates a transient accumulation of dephosphorylated β-catenin in the cytoplasm and its translocation to the nucleus. This effect of strain acts through activation of protein kinase B and phosphorylation of glycogen synthase kinase-3 beta. These strain-related changes do not involve the low-density lipoprotein receptor-related protein 5/Wnt receptor. Conclusions: The Wnt/β-catenin signaling pathway components are functional and activated by mechanical loading in PDL cells. β-catenin serves as an effector of mechanical signals in PDL cells.",
keywords = "Mechanical loading, Periodontal ligament cells, Tooth movement, β-catenin",
author = "Sundaralingam Premaraj and Isabela Souza and Thyagaseely Premaraj",
year = "2011",
month = "7",
day = "1",
doi = "10.2319/090310-519.1",
language = "English (US)",
volume = "81",
pages = "592--599",
journal = "Angle Orthodontist",
issn = "0003-3219",
publisher = "E H Angle Orthodontists Research & Education Foundation, Inc.",
number = "4",

}

TY - JOUR

T1 - Mechanical loading activates β-catenin signaling in periodontal ligament cells

AU - Premaraj, Sundaralingam

AU - Souza, Isabela

AU - Premaraj, Thyagaseely

PY - 2011/7/1

Y1 - 2011/7/1

N2 - Objective: To determine whether β-catenin signaling is responsive to mechanical loading in periodontal ligament (PDL) cells. Materials and Methods: To determine whether Wnt/β-catenin signaling pathway components are present and functional, PDL cells were treated with lithium chloride or Wnt3a-conditioned media. To determine whether mechanical strain activates β-catenin signaling, PDL cells were subjected to compressive loading. Activation of the β-catenin signaling pathway was determined by immunofluorescence, Western immunoblotting, and TOPflash assay. Results: Mimicking Wnt signaling stimulates β-catenin nuclear translocation and T-cell factor/ lymphoid enhancer binding factor-dependent transcriptional activation in PDL cells. Mechanical loading stimulates a transient accumulation of dephosphorylated β-catenin in the cytoplasm and its translocation to the nucleus. This effect of strain acts through activation of protein kinase B and phosphorylation of glycogen synthase kinase-3 beta. These strain-related changes do not involve the low-density lipoprotein receptor-related protein 5/Wnt receptor. Conclusions: The Wnt/β-catenin signaling pathway components are functional and activated by mechanical loading in PDL cells. β-catenin serves as an effector of mechanical signals in PDL cells.

AB - Objective: To determine whether β-catenin signaling is responsive to mechanical loading in periodontal ligament (PDL) cells. Materials and Methods: To determine whether Wnt/β-catenin signaling pathway components are present and functional, PDL cells were treated with lithium chloride or Wnt3a-conditioned media. To determine whether mechanical strain activates β-catenin signaling, PDL cells were subjected to compressive loading. Activation of the β-catenin signaling pathway was determined by immunofluorescence, Western immunoblotting, and TOPflash assay. Results: Mimicking Wnt signaling stimulates β-catenin nuclear translocation and T-cell factor/ lymphoid enhancer binding factor-dependent transcriptional activation in PDL cells. Mechanical loading stimulates a transient accumulation of dephosphorylated β-catenin in the cytoplasm and its translocation to the nucleus. This effect of strain acts through activation of protein kinase B and phosphorylation of glycogen synthase kinase-3 beta. These strain-related changes do not involve the low-density lipoprotein receptor-related protein 5/Wnt receptor. Conclusions: The Wnt/β-catenin signaling pathway components are functional and activated by mechanical loading in PDL cells. β-catenin serves as an effector of mechanical signals in PDL cells.

KW - Mechanical loading

KW - Periodontal ligament cells

KW - Tooth movement

KW - β-catenin

UR - http://www.scopus.com/inward/record.url?scp=79960185777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960185777&partnerID=8YFLogxK

U2 - 10.2319/090310-519.1

DO - 10.2319/090310-519.1

M3 - Article

VL - 81

SP - 592

EP - 599

JO - Angle Orthodontist

JF - Angle Orthodontist

SN - 0003-3219

IS - 4

ER -