Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering

Chiara Rinoldi, Afsoon Fallahi, Iman K. Yazdi, Jessica Campos Paras, Ewa Kijeńska-Gawrońska, Grissel Trujillo-De Santiago, Abuduwaili Tuoheti, Danilo Demarchi, Nasim Annabi, Ali Khademhosseini, Wojciech Swieszkowski, Ali Tamayol

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tendon injuries are frequent and occur in the elderly, young, and athletic populations. The inadequate number of donors combined with many challenges associated with autografts, allografts, xenografts, and prosthetic devices have added to the value of engineering biological substitutes, which can be implanted to repair the damaged tendons. Electrospun scaffolds have the potential to mimic the native tissue structure along with desired mechanical properties and, thus, have attracted noticeable attention. In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel. The whole construct composition was optimized to achieve adequate mechanical and physical properties as well as cell viability and proliferation. Mesenchymal stem cells (MSCs) were differentiated by the addition of bone morphogenetic protein 12 (BMP-12). To mimic the natural function of tendons, the cell-laden scaffolds were mechanically stimulated using a custom-built bioreactor. The synergistic effect of mechanical and biochemical stimulation was observed in terms of enhanced cell viability, proliferation, alignment, and tenogenic differentiation. The results suggested that the proposed constructs can be used for engineering functional tendons.

Original languageEnglish (US)
Pages (from-to)2953-2964
Number of pages12
JournalACS Biomaterials Science and Engineering
Volume5
Issue number6
DOIs
StatePublished - Jun 10 2019

Fingerprint

Tendons
Scaffolds (biology)
Tissue engineering
Scaffolds
Mechanical properties
Hydrogel
Autografts
Cell proliferation
Prosthetics
Bioreactors
Stem cells
Heterografts
Hydrogels
Allografts
Bone
Repair
Physical properties
Cells
Tissue
Proteins

Keywords

  • composite scaffolds
  • mechanical stimulation
  • nanofibrous materials
  • stem cell differentiation
  • tendon tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Rinoldi, C., Fallahi, A., Yazdi, I. K., Campos Paras, J., Kijeńska-Gawrońska, E., Trujillo-De Santiago, G., ... Tamayol, A. (2019). Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering. ACS Biomaterials Science and Engineering, 5(6), 2953-2964. https://doi.org/10.1021/acsbiomaterials.8b01647

Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering. / Rinoldi, Chiara; Fallahi, Afsoon; Yazdi, Iman K.; Campos Paras, Jessica; Kijeńska-Gawrońska, Ewa; Trujillo-De Santiago, Grissel; Tuoheti, Abuduwaili; Demarchi, Danilo; Annabi, Nasim; Khademhosseini, Ali; Swieszkowski, Wojciech; Tamayol, Ali.

In: ACS Biomaterials Science and Engineering, Vol. 5, No. 6, 10.06.2019, p. 2953-2964.

Research output: Contribution to journalArticle

Rinoldi, C, Fallahi, A, Yazdi, IK, Campos Paras, J, Kijeńska-Gawrońska, E, Trujillo-De Santiago, G, Tuoheti, A, Demarchi, D, Annabi, N, Khademhosseini, A, Swieszkowski, W & Tamayol, A 2019, 'Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering', ACS Biomaterials Science and Engineering, vol. 5, no. 6, pp. 2953-2964. https://doi.org/10.1021/acsbiomaterials.8b01647
Rinoldi C, Fallahi A, Yazdi IK, Campos Paras J, Kijeńska-Gawrońska E, Trujillo-De Santiago G et al. Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering. ACS Biomaterials Science and Engineering. 2019 Jun 10;5(6):2953-2964. https://doi.org/10.1021/acsbiomaterials.8b01647
Rinoldi, Chiara ; Fallahi, Afsoon ; Yazdi, Iman K. ; Campos Paras, Jessica ; Kijeńska-Gawrońska, Ewa ; Trujillo-De Santiago, Grissel ; Tuoheti, Abuduwaili ; Demarchi, Danilo ; Annabi, Nasim ; Khademhosseini, Ali ; Swieszkowski, Wojciech ; Tamayol, Ali. / Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering. In: ACS Biomaterials Science and Engineering. 2019 ; Vol. 5, No. 6. pp. 2953-2964.
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