MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB

Jie Chao, Xiaoniu Dai, Tiffany Peña, David A. Doyle, Timothy M. Guenther, Mark Alan Carlson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to model matrix contraction, cell motility, and general fibroblast biology. MCPIP1 (monocyte chemotactic protein-induced protein 1) has been shown to regulate inflammation, angiogenesis, and cellular motility. In the present study, we demonstrated induction of MCPIP1 in human fibroblasts embedded in the stress-released 3-D collagen matrix, which occurred through activation of mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-κB. Furthermore, MCPIP1 induction was associated with inhibition of fibroblast migration out of the nested collagen matrix. MCPIP1 induction or ectopic expression also upregulated p53. RNA interference of p53 prevented the inhibition of migration produced by induction or ectopic expression of MCPIP1. Our findings suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast migration in the nested collagen matrix model.

Original languageEnglish (US)
Pages (from-to)2944-2954
Number of pages11
JournalJournal of Investigative Dermatology
Volume135
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Monocyte Chemoattractant Proteins
Fibroblasts
Phosphotransferases
Collagen
Proteins
MAP Kinase Kinase 3
1-Phosphatidylinositol 4-Kinase
Phosphatidylinositols
RNA Interference
Mitogen-Activated Protein Kinases
Cell Movement
Chemical activation
Switches
RNA
Inflammation

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB. / Chao, Jie; Dai, Xiaoniu; Peña, Tiffany; Doyle, David A.; Guenther, Timothy M.; Carlson, Mark Alan.

In: Journal of Investigative Dermatology, Vol. 135, No. 12, 01.12.2015, p. 2944-2954.

Research output: Contribution to journalArticle

Chao, Jie ; Dai, Xiaoniu ; Peña, Tiffany ; Doyle, David A. ; Guenther, Timothy M. ; Carlson, Mark Alan. / MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB. In: Journal of Investigative Dermatology. 2015 ; Vol. 135, No. 12. pp. 2944-2954.
@article{36735613d356443aba45fa2266ed9da3,
title = "MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB",
abstract = "The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to model matrix contraction, cell motility, and general fibroblast biology. MCPIP1 (monocyte chemotactic protein-induced protein 1) has been shown to regulate inflammation, angiogenesis, and cellular motility. In the present study, we demonstrated induction of MCPIP1 in human fibroblasts embedded in the stress-released 3-D collagen matrix, which occurred through activation of mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-κB. Furthermore, MCPIP1 induction was associated with inhibition of fibroblast migration out of the nested collagen matrix. MCPIP1 induction or ectopic expression also upregulated p53. RNA interference of p53 prevented the inhibition of migration produced by induction or ectopic expression of MCPIP1. Our findings suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast migration in the nested collagen matrix model.",
author = "Jie Chao and Xiaoniu Dai and Tiffany Pe{\~n}a and Doyle, {David A.} and Guenther, {Timothy M.} and Carlson, {Mark Alan}",
year = "2015",
month = "12",
day = "1",
doi = "10.1038/jid.2015.334",
language = "English (US)",
volume = "135",
pages = "2944--2954",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB

AU - Chao, Jie

AU - Dai, Xiaoniu

AU - Peña, Tiffany

AU - Doyle, David A.

AU - Guenther, Timothy M.

AU - Carlson, Mark Alan

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to model matrix contraction, cell motility, and general fibroblast biology. MCPIP1 (monocyte chemotactic protein-induced protein 1) has been shown to regulate inflammation, angiogenesis, and cellular motility. In the present study, we demonstrated induction of MCPIP1 in human fibroblasts embedded in the stress-released 3-D collagen matrix, which occurred through activation of mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-κB. Furthermore, MCPIP1 induction was associated with inhibition of fibroblast migration out of the nested collagen matrix. MCPIP1 induction or ectopic expression also upregulated p53. RNA interference of p53 prevented the inhibition of migration produced by induction or ectopic expression of MCPIP1. Our findings suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast migration in the nested collagen matrix model.

AB - The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to model matrix contraction, cell motility, and general fibroblast biology. MCPIP1 (monocyte chemotactic protein-induced protein 1) has been shown to regulate inflammation, angiogenesis, and cellular motility. In the present study, we demonstrated induction of MCPIP1 in human fibroblasts embedded in the stress-released 3-D collagen matrix, which occurred through activation of mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-κB. Furthermore, MCPIP1 induction was associated with inhibition of fibroblast migration out of the nested collagen matrix. MCPIP1 induction or ectopic expression also upregulated p53. RNA interference of p53 prevented the inhibition of migration produced by induction or ectopic expression of MCPIP1. Our findings suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast migration in the nested collagen matrix model.

UR - http://www.scopus.com/inward/record.url?scp=84948713826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84948713826&partnerID=8YFLogxK

U2 - 10.1038/jid.2015.334

DO - 10.1038/jid.2015.334

M3 - Article

VL - 135

SP - 2944

EP - 2954

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 12

ER -