Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of titanium oxide-enriched peptides for detection of aged organophosphorus adducts on human butyrylcholinesterase

Wei Jiang, Ekaterina A. Murashko, Yaroslav A. Dubrovskii, Ekaterina P. Podolskaya, Vladimir N. Babakov, John Mikler, Florian Nachon, Patrick Masson, Lawrence M Schopfer, Oksana Lockridge

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Exposure to nerve agents or organophosphorus (OP) pesticides can have life-threatening effects. Human plasma butyrylcholinesterase (BChE) inactivates these poisons by binding them to Ser198. After hours or days, these OP adducts acquire a negative charge by dealkylation in a process called aging. Our goal was to develop a method for enriching the aged adduct to facilitate detection of exposure. Human BChE inhibited by OP toxicants was incubated for 4 days to 6 years. Peptides produced by digestion with pepsin were enriched by binding to titanium oxide (TiO2) and analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. It was found that with two exceptions, all aged OP adducts in peptide FGES 198AGAAS were enriched by binding to Titansphere tips. Cresyl saligenin phosphate yielded two types of aged adduct, cresylphosphate and phosphate, but only the phosphate adduct bound to Titansphere. The nerve agent VR yielded no aged adduct, supporting crystal structure findings that the VR adduct on BChE does not age. The irreversible nature of aged OP adducts was demonstrated by the finding that after 6 years at room temperature in sterile pH 7.0 buffer, the adducts were still detectable. It was concluded that TiO 2 microcolumns can be used to enrich aged OP-modified BChE peptide.

Original languageEnglish (US)
Pages (from-to)132-141
Number of pages10
JournalAnalytical Biochemistry
Volume439
Issue number2
DOIs
Publication statusPublished - Jan 1 2013

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Keywords

  • Aged organophosphorus adducts
  • Human butyrylcholinesterase
  • Mass spectrometry
  • Titanium oxide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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