Maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancy through metabolic stress and mitochondrial dysfunction

Kennedy S. Mdaki, Tricia D. Larsen, Angela L. Wachal, Michelle D. Schimelpfenig, Lucinda J. Weaver, Samuel D.R. Dooyema, Eli J. Louwagie, Michelle Baack

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Offspring of diabetic pregnancies are at risk of cardiovascular disease at birth and throughout life, purportedly through fuel-mediated influences on the developing heart. Preventative measures focus on glycemic control, but the contribution of additional offenders, including lipids, is not understood. Cellular bioenergetics can be influenced by both diabetes and hyperlipidemia and play a pivotal role in the pathophysiology of adult cardiovascular disease. This study investigated whether a maternal high-fat diet, independently or additively with diabetes, could impair fuel metabolism, mitochondrial function, and cardiac physiology in the developing offspring's heart. Sprague-Dawley rats fed a control or high-fat diet were administered placebo or streptozotocin to induce diabetes during pregnancy and then delivered offspring from four groups: control, diabetes exposed, diet exposed, and combination exposed. Cardiac function, cellular bioenergetics (mitochondrial stress test, glycolytic stress test, and palmitate oxidation assay), lipid peroxidation, mitochondrial histology, and copy number were determined. Diabetes-exposed offspring had impaired glycolytic and respiratory capacity and a reduced proton leak. High-fat diet-exposed offspring had increased mitochondrial copy number, increased lipid peroxidation, and evidence of mitochondrial dysfunction. Combination-exposed pups were most severely affected and demonstrated cardiac lipid droplet accumulation and diastolic/systolic cardiac dysfunction that mimics that of adult diabetic cardiomyopathy. This study is the first to demonstrate that a maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancies through metabolic stress and serves as a critical step in understanding the role of cellular bioenergetics in developmentally programmed cardiac disease.

Original languageEnglish (US)
Pages (from-to)H681-H692
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume310
Issue number6
DOIs
StatePublished - Mar 1 2016

Fingerprint

Pregnancy in Diabetics
Physiological Stress
High Fat Diet
Mothers
Energy Metabolism
Exercise Test
Lipid Peroxidation
Cardiovascular Diseases
Diabetic Cardiomyopathies
Pregnancy
Palmitates
Streptozocin
Hyperlipidemias
Sprague Dawley Rats
Protons
Heart Diseases
Histology
Placebos
Parturition
Diet

Keywords

  • Cardiac bioenergetics
  • Developmental programming
  • Diabetic cardiomyopathy
  • Diabetic pregnancy
  • Maternal high-fat diet
  • Mitochondrial dysfunction
  • Proton production rate

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancy through metabolic stress and mitochondrial dysfunction. / Mdaki, Kennedy S.; Larsen, Tricia D.; Wachal, Angela L.; Schimelpfenig, Michelle D.; Weaver, Lucinda J.; Dooyema, Samuel D.R.; Louwagie, Eli J.; Baack, Michelle.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 310, No. 6, 01.03.2016, p. H681-H692.

Research output: Contribution to journalArticle

Mdaki, Kennedy S. ; Larsen, Tricia D. ; Wachal, Angela L. ; Schimelpfenig, Michelle D. ; Weaver, Lucinda J. ; Dooyema, Samuel D.R. ; Louwagie, Eli J. ; Baack, Michelle. / Maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancy through metabolic stress and mitochondrial dysfunction. In: American Journal of Physiology - Heart and Circulatory Physiology. 2016 ; Vol. 310, No. 6. pp. H681-H692.
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