Mass spectrometric methods

an answer for macromolecule analysis in the 1990s

Michael L. Gross, Ronald Cerny, Daryl E. Giblin, Don L. Rempel, Denise K. MacMillan, Peifeng Hu, Christopher L. Holliman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The field of mass spectrometry is now well developed for solving analytical and structural problems involving substances with molecular weights less than ca. 1000. The future challenge for mass spectrometry is in the area of macromolecule analysis and structural biology. This challenge will be met on two fronts. One is structural analysis of pieces of macromolecules, a task for tandem mass spectrometers. Tandem sector instruments offer sufficient control, reproducibility of results and ease of set-up that they will play a major role in structure studies. When designed to operate with extended array detectors, tandem sector instruments will also offer subpicomole detection limits. The second front is molecular weight measurements. Exciting advances include matrix-assisted laser desorption and electrospray ionization. The need for better means of mass analysis is forecast, and it is suggested that the Fourier transform mass spectrometer can meet the challenge. Success awaits a better understanding of the dynamics of high-mass ions. One route to improved understanding is outlined.

Original languageEnglish (US)
Pages (from-to)105-130
Number of pages26
JournalAnalytica Chimica Acta
Volume250
Issue numberC
DOIs
StatePublished - Oct 1 1991

Fingerprint

Mass spectrometers
Macromolecules
Mass spectrometry
Mass Spectrometry
Molecular Weight
Molecular weight
Electrospray ionization
Weighing
Fourier Analysis
Reproducibility of Results
Structural analysis
Limit of Detection
Desorption
Fourier transforms
Lasers
spectrometer
mass spectrometry
Ions
Detectors
structural analysis

Keywords

  • Biological samples
  • Fourier transform
  • Macromolecules
  • Mass spectrometry
  • Tandem sector mass spectrometers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Gross, M. L., Cerny, R., Giblin, D. E., Rempel, D. L., MacMillan, D. K., Hu, P., & Holliman, C. L. (1991). Mass spectrometric methods: an answer for macromolecule analysis in the 1990s. Analytica Chimica Acta, 250(C), 105-130. https://doi.org/10.1016/0003-2670(91)85066-2

Mass spectrometric methods : an answer for macromolecule analysis in the 1990s. / Gross, Michael L.; Cerny, Ronald; Giblin, Daryl E.; Rempel, Don L.; MacMillan, Denise K.; Hu, Peifeng; Holliman, Christopher L.

In: Analytica Chimica Acta, Vol. 250, No. C, 01.10.1991, p. 105-130.

Research output: Contribution to journalArticle

Gross, ML, Cerny, R, Giblin, DE, Rempel, DL, MacMillan, DK, Hu, P & Holliman, CL 1991, 'Mass spectrometric methods: an answer for macromolecule analysis in the 1990s', Analytica Chimica Acta, vol. 250, no. C, pp. 105-130. https://doi.org/10.1016/0003-2670(91)85066-2
Gross, Michael L. ; Cerny, Ronald ; Giblin, Daryl E. ; Rempel, Don L. ; MacMillan, Denise K. ; Hu, Peifeng ; Holliman, Christopher L. / Mass spectrometric methods : an answer for macromolecule analysis in the 1990s. In: Analytica Chimica Acta. 1991 ; Vol. 250, No. C. pp. 105-130.
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