Mapping the Cell-Surface N-Glycoproteome of Human Hepatocytes Reveals Markers for Selecting a Homogeneous Population of iPSC-Derived Hepatocytes

Sunil K. Mallanna, Max A. Cayo, Kirk Twaroski, Rebekah L. Gundry, Stephen A. Duncan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

When comparing hepatic phenotypes between iPSC-derived hepatocyte-like cells from different liver disease patients, cell heterogeneity can confound interpretation. We proposed that homogeneous cell populations could be generated by fluorescence-activated cell sorting (FACS). Using cell-surface capture proteomics, we identified a total of 300 glycoproteins on hepatocytes. Analyses of the expression profiles during the differentiation of iPSCs revealed that SLC10A1, CLRN3, and AADAC were highly enriched during the final stages of hepatocyte differentiation. FACS purification of hepatocyte-like cells expressing SLC10A1, CLRN3, or AADAC demonstrated enrichment of cells with hepatocyte characteristics. Moreover, transcriptome analyses revealed that cells expressing the liver gene regulatory network were enriched while cells expressing a pluripotent stem cell network were depleted. In conclusion, we report an extensive catalog of cell-surface N-linked glycoproteins expressed in primary hepatocytes and identify cell-surface proteins that facilitate the purification of homogeneous populations of iPSC-derived hepatocyte-like cells.

Original languageEnglish (US)
Pages (from-to)543-556
Number of pages14
JournalStem Cell Reports
Volume7
Issue number3
DOIs
StatePublished - Sep 13 2016

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Hepatocytes
Cells
Population
Sorting
Liver
Purification
Glycoproteins
Fluorescence
Flow Cytometry
Stem cells
Pluripotent Stem Cells
Membrane Proteins
Gene Regulatory Networks
Genes
Gene Expression Profiling
Proteomics
Liver Diseases
Phenotype

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Mapping the Cell-Surface N-Glycoproteome of Human Hepatocytes Reveals Markers for Selecting a Homogeneous Population of iPSC-Derived Hepatocytes. / Mallanna, Sunil K.; Cayo, Max A.; Twaroski, Kirk; Gundry, Rebekah L.; Duncan, Stephen A.

In: Stem Cell Reports, Vol. 7, No. 3, 13.09.2016, p. 543-556.

Research output: Contribution to journalArticle

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