Malonate: A versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals

Todd Holyoak, Timothy D. Fenn, Mark A. Wilson, Aaron G. Moulin, Dagmar Ringe, Gregory A. Petsko

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.

Original languageEnglish (US)
Pages (from-to)2356-2358
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number12
DOIs
StatePublished - Dec 1 2003

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Salts
Sodium
salts
Crystals
Synchrotrons
Ice
crystals
Proteins
Ligands
sodium
proteins
ice formation
soaking
manipulators
synchrotrons
malonic acid
retarding
damage
ligands
Data Accuracy

ASJC Scopus subject areas

  • Structural Biology

Cite this

Malonate : A versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. / Holyoak, Todd; Fenn, Timothy D.; Wilson, Mark A.; Moulin, Aaron G.; Ringe, Dagmar; Petsko, Gregory A.

In: Acta Crystallographica - Section D Biological Crystallography, Vol. 59, No. 12, 01.12.2003, p. 2356-2358.

Research output: Contribution to journalArticle

Holyoak, Todd ; Fenn, Timothy D. ; Wilson, Mark A. ; Moulin, Aaron G. ; Ringe, Dagmar ; Petsko, Gregory A. / Malonate : A versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. In: Acta Crystallographica - Section D Biological Crystallography. 2003 ; Vol. 59, No. 12. pp. 2356-2358.
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