Malnutrition impairs postresection intestinal adaptation

D. R. Cronk, D. C. Ferguson, J. S. Thompson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Postresection intestinal adaptation is influenced by several factors, including luminal nutrients. Adaptation is impaired in the absence of luminal nutrients, even if the nutrition is maintained via total parenteral nutrition (TPN). Reduced enteral intake also inhibits adaptation if malnutrition is present, but the mechanism has not been completely defined. Our aim was to study the effect of reduced enteral nutrition on adaptation and enterocyte production and death after 50% proximal resection in rats. Methods: Eighteen Lewis rats underwent either transection (n =6) or 50% proximal resection (n =12). The resected animals either ate ad libitum (n =6) or were offered 75% of ad libitum intake (n =6). Nutritional status and intestinal adaptation were determined 14 days after surgery. Results: Resected animals receiving 75% normal intake had decreased body weight (89% ± 4% vs 112% ± 2% and 112% ± 1%, p < .05) and serum albumin (2.7 ± 0.1 vs 3.2 ± 0.0 g/dL and 3.0 ± 0.1 g/dL, p < .05) compared with resection with normal intake and transection, respectively. Intestinal weight (0.32 ± 03 vs 0.22 ± 0.02 g/cm and 0.19 ± 0.03 g/cm, p < .05) and diameter (10.5 ± 0.5 vs 8.5 ± 1.0 mm and 7.8 ± 0.8 mm, p < .05) increased after resection alone compared with transection and malnourished resection groups. Gut weight and diameter and villus height were lower in malnourished resected animals than with transection. Crypt cell production rate was significantly lower in the reduced intake animals. Apoptosis was increased in both crypt and villus enterocytes in normally nourished but not malnourished resected animals. Villus apoptosis correlated with villus height. Conclusions: Intestinal adaptation is impaired by a 25% reduction in enteral nutrients, confirming that both the route and quantity of nutrient intake are important in this process. Both enterocyte production and loss via apoptosis are decreased by reduced enteral intake and malnutrition after resection. The correlation between villus height and apoptosis suggests that the reduced apoptosis reflects the smaller enterocyte number in malnourished animals rather than an adaptive response to maintain intestinal structure.

Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalJournal of Parenteral and Enteral Nutrition
Volume24
Issue number2
DOIs
StatePublished - Jan 1 2000

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resection
Malnutrition
malnutrition
villi
Enterocytes
enterocytes
apoptosis
Apoptosis
Small Intestine
Food
animals
nutrients
Weights and Measures
Total Parenteral Nutrition
total parenteral nutrition
rats
enteral feeding
Enteral Nutrition
serum albumin
Nutritional Status

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Malnutrition impairs postresection intestinal adaptation. / Cronk, D. R.; Ferguson, D. C.; Thompson, J. S.

In: Journal of Parenteral and Enteral Nutrition, Vol. 24, No. 2, 01.01.2000, p. 76-80.

Research output: Contribution to journalArticle

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abstract = "Background: Postresection intestinal adaptation is influenced by several factors, including luminal nutrients. Adaptation is impaired in the absence of luminal nutrients, even if the nutrition is maintained via total parenteral nutrition (TPN). Reduced enteral intake also inhibits adaptation if malnutrition is present, but the mechanism has not been completely defined. Our aim was to study the effect of reduced enteral nutrition on adaptation and enterocyte production and death after 50{\%} proximal resection in rats. Methods: Eighteen Lewis rats underwent either transection (n =6) or 50{\%} proximal resection (n =12). The resected animals either ate ad libitum (n =6) or were offered 75{\%} of ad libitum intake (n =6). Nutritional status and intestinal adaptation were determined 14 days after surgery. Results: Resected animals receiving 75{\%} normal intake had decreased body weight (89{\%} ± 4{\%} vs 112{\%} ± 2{\%} and 112{\%} ± 1{\%}, p < .05) and serum albumin (2.7 ± 0.1 vs 3.2 ± 0.0 g/dL and 3.0 ± 0.1 g/dL, p < .05) compared with resection with normal intake and transection, respectively. Intestinal weight (0.32 ± 03 vs 0.22 ± 0.02 g/cm and 0.19 ± 0.03 g/cm, p < .05) and diameter (10.5 ± 0.5 vs 8.5 ± 1.0 mm and 7.8 ± 0.8 mm, p < .05) increased after resection alone compared with transection and malnourished resection groups. Gut weight and diameter and villus height were lower in malnourished resected animals than with transection. Crypt cell production rate was significantly lower in the reduced intake animals. Apoptosis was increased in both crypt and villus enterocytes in normally nourished but not malnourished resected animals. Villus apoptosis correlated with villus height. Conclusions: Intestinal adaptation is impaired by a 25{\%} reduction in enteral nutrients, confirming that both the route and quantity of nutrient intake are important in this process. Both enterocyte production and loss via apoptosis are decreased by reduced enteral intake and malnutrition after resection. The correlation between villus height and apoptosis suggests that the reduced apoptosis reflects the smaller enterocyte number in malnourished animals rather than an adaptive response to maintain intestinal structure.",
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AU - Cronk, D. R.

AU - Ferguson, D. C.

AU - Thompson, J. S.

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N2 - Background: Postresection intestinal adaptation is influenced by several factors, including luminal nutrients. Adaptation is impaired in the absence of luminal nutrients, even if the nutrition is maintained via total parenteral nutrition (TPN). Reduced enteral intake also inhibits adaptation if malnutrition is present, but the mechanism has not been completely defined. Our aim was to study the effect of reduced enteral nutrition on adaptation and enterocyte production and death after 50% proximal resection in rats. Methods: Eighteen Lewis rats underwent either transection (n =6) or 50% proximal resection (n =12). The resected animals either ate ad libitum (n =6) or were offered 75% of ad libitum intake (n =6). Nutritional status and intestinal adaptation were determined 14 days after surgery. Results: Resected animals receiving 75% normal intake had decreased body weight (89% ± 4% vs 112% ± 2% and 112% ± 1%, p < .05) and serum albumin (2.7 ± 0.1 vs 3.2 ± 0.0 g/dL and 3.0 ± 0.1 g/dL, p < .05) compared with resection with normal intake and transection, respectively. Intestinal weight (0.32 ± 03 vs 0.22 ± 0.02 g/cm and 0.19 ± 0.03 g/cm, p < .05) and diameter (10.5 ± 0.5 vs 8.5 ± 1.0 mm and 7.8 ± 0.8 mm, p < .05) increased after resection alone compared with transection and malnourished resection groups. Gut weight and diameter and villus height were lower in malnourished resected animals than with transection. Crypt cell production rate was significantly lower in the reduced intake animals. Apoptosis was increased in both crypt and villus enterocytes in normally nourished but not malnourished resected animals. Villus apoptosis correlated with villus height. Conclusions: Intestinal adaptation is impaired by a 25% reduction in enteral nutrients, confirming that both the route and quantity of nutrient intake are important in this process. Both enterocyte production and loss via apoptosis are decreased by reduced enteral intake and malnutrition after resection. The correlation between villus height and apoptosis suggests that the reduced apoptosis reflects the smaller enterocyte number in malnourished animals rather than an adaptive response to maintain intestinal structure.

AB - Background: Postresection intestinal adaptation is influenced by several factors, including luminal nutrients. Adaptation is impaired in the absence of luminal nutrients, even if the nutrition is maintained via total parenteral nutrition (TPN). Reduced enteral intake also inhibits adaptation if malnutrition is present, but the mechanism has not been completely defined. Our aim was to study the effect of reduced enteral nutrition on adaptation and enterocyte production and death after 50% proximal resection in rats. Methods: Eighteen Lewis rats underwent either transection (n =6) or 50% proximal resection (n =12). The resected animals either ate ad libitum (n =6) or were offered 75% of ad libitum intake (n =6). Nutritional status and intestinal adaptation were determined 14 days after surgery. Results: Resected animals receiving 75% normal intake had decreased body weight (89% ± 4% vs 112% ± 2% and 112% ± 1%, p < .05) and serum albumin (2.7 ± 0.1 vs 3.2 ± 0.0 g/dL and 3.0 ± 0.1 g/dL, p < .05) compared with resection with normal intake and transection, respectively. Intestinal weight (0.32 ± 03 vs 0.22 ± 0.02 g/cm and 0.19 ± 0.03 g/cm, p < .05) and diameter (10.5 ± 0.5 vs 8.5 ± 1.0 mm and 7.8 ± 0.8 mm, p < .05) increased after resection alone compared with transection and malnourished resection groups. Gut weight and diameter and villus height were lower in malnourished resected animals than with transection. Crypt cell production rate was significantly lower in the reduced intake animals. Apoptosis was increased in both crypt and villus enterocytes in normally nourished but not malnourished resected animals. Villus apoptosis correlated with villus height. Conclusions: Intestinal adaptation is impaired by a 25% reduction in enteral nutrients, confirming that both the route and quantity of nutrient intake are important in this process. Both enterocyte production and loss via apoptosis are decreased by reduced enteral intake and malnutrition after resection. The correlation between villus height and apoptosis suggests that the reduced apoptosis reflects the smaller enterocyte number in malnourished animals rather than an adaptive response to maintain intestinal structure.

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