Maize pyruvate decarboxylase mRNA is induced anaerobically

Philip M. Kelley

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

Original languageEnglish (US)
Pages (from-to)213-222
Number of pages10
JournalPlant Molecular Biology
Volume13
Issue number2
DOIs
Publication statusPublished - Aug 1 1989

    Fingerprint

Keywords

  • anaerobic stress
  • gene regulation
  • maize
  • plant
  • pyruvate decarboxylase
  • sequence

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this