Maize pyruvate decarboxylase mRNA is induced anaerobically

Philip M. Kelley

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

Original languageEnglish (US)
Pages (from-to)213-222
Number of pages10
JournalPlant Molecular Biology
Volume13
Issue number2
DOIs
StatePublished - Aug 1 1989

Fingerprint

Pyruvate Decarboxylase
pyruvate decarboxylase
Oligonucleotides
Yeast
Zea mays
Complementary DNA
oligonucleotides
Messenger RNA
corn
Yeasts
yeasts
Immune Sera
Proteins
Genes
Southern Blotting
Northern blotting
Northern Blotting
Southern blotting
Open Reading Frames
antiserum

Keywords

  • anaerobic stress
  • gene regulation
  • maize
  • plant
  • pyruvate decarboxylase
  • sequence

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Maize pyruvate decarboxylase mRNA is induced anaerobically. / Kelley, Philip M.

In: Plant Molecular Biology, Vol. 13, No. 2, 01.08.1989, p. 213-222.

Research output: Contribution to journalArticle

Kelley, Philip M. / Maize pyruvate decarboxylase mRNA is induced anaerobically. In: Plant Molecular Biology. 1989 ; Vol. 13, No. 2. pp. 213-222.
@article{82ae229c8da64b3a9b51c12bc9025718,
title = "Maize pyruvate decarboxylase mRNA is induced anaerobically",
abstract = "A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.",
keywords = "anaerobic stress, gene regulation, maize, plant, pyruvate decarboxylase, sequence",
author = "Kelley, {Philip M.}",
year = "1989",
month = "8",
day = "1",
doi = "10.1007/BF00016139",
language = "English (US)",
volume = "13",
pages = "213--222",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Maize pyruvate decarboxylase mRNA is induced anaerobically

AU - Kelley, Philip M.

PY - 1989/8/1

Y1 - 1989/8/1

N2 - A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

AB - A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

KW - anaerobic stress

KW - gene regulation

KW - maize

KW - plant

KW - pyruvate decarboxylase

KW - sequence

UR - http://www.scopus.com/inward/record.url?scp=0024716825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024716825&partnerID=8YFLogxK

U2 - 10.1007/BF00016139

DO - 10.1007/BF00016139

M3 - Article

C2 - 2519113

AN - SCOPUS:0024716825

VL - 13

SP - 213

EP - 222

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 2

ER -