Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling

Sumitra Bhattacharya, Ani Das, Kavita Mallya, Iqbal Ahmad

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

ABCG2 belongs to the ATP-binding cassette superfamily of transmembrane proteins and is ubiquitously expressed in stem cells including those in the developing nervous system. The ability of ABCG2 to preferentially exclude DNA-intercalating dyes is regarded to be the basis for the enrichment of stem cells or progenitors as dyelow side population (SP) cells. However, the role of ABCG2 in neural stem cells remains speculative and poorly understood. Here, we demonstrate using retinal stem cells, that ABCG2 is the molecular determinant of SP cell phenotype of neural stem cells and plays an important role in their maintenance. Overexpression of ABCG2 prevents the SP cell phenotype and adversely affects the lineage commitment of retinal stem cells. By contrast, targeted attenuation of ABCG2 depletes retinal SP cells and promotes their differentiation along pan neural and retinal lineages. In addition, we demonstrate for the first time that ABCG2 is a target of Notch signaling, and as such, constitutes one of the genes in the regulatory network of Notch signaling, involved in the maintenance of stem cells.

Original languageEnglish (US)
Pages (from-to)2652-2662
Number of pages11
JournalJournal of Cell Science
Volume120
Issue number15
DOIs
StatePublished - Aug 1 2007

Fingerprint

Side-Population Cells
Stem Cells
Maintenance
Neural Stem Cells
Phenotype
Gene Regulatory Networks
Nervous System
Coloring Agents
Adenosine Triphosphate
DNA
Proteins

Keywords

  • ABCG2
  • Notch
  • Progenitors
  • Retina
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling. / Bhattacharya, Sumitra; Das, Ani; Mallya, Kavita; Ahmad, Iqbal.

In: Journal of Cell Science, Vol. 120, No. 15, 01.08.2007, p. 2652-2662.

Research output: Contribution to journalArticle

Bhattacharya, Sumitra ; Das, Ani ; Mallya, Kavita ; Ahmad, Iqbal. / Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling. In: Journal of Cell Science. 2007 ; Vol. 120, No. 15. pp. 2652-2662.
@article{6a71ff465c434532be69e1a64c8d5123,
title = "Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling",
abstract = "ABCG2 belongs to the ATP-binding cassette superfamily of transmembrane proteins and is ubiquitously expressed in stem cells including those in the developing nervous system. The ability of ABCG2 to preferentially exclude DNA-intercalating dyes is regarded to be the basis for the enrichment of stem cells or progenitors as dyelow side population (SP) cells. However, the role of ABCG2 in neural stem cells remains speculative and poorly understood. Here, we demonstrate using retinal stem cells, that ABCG2 is the molecular determinant of SP cell phenotype of neural stem cells and plays an important role in their maintenance. Overexpression of ABCG2 prevents the SP cell phenotype and adversely affects the lineage commitment of retinal stem cells. By contrast, targeted attenuation of ABCG2 depletes retinal SP cells and promotes their differentiation along pan neural and retinal lineages. In addition, we demonstrate for the first time that ABCG2 is a target of Notch signaling, and as such, constitutes one of the genes in the regulatory network of Notch signaling, involved in the maintenance of stem cells.",
keywords = "ABCG2, Notch, Progenitors, Retina, Stem cells",
author = "Sumitra Bhattacharya and Ani Das and Kavita Mallya and Iqbal Ahmad",
year = "2007",
month = "8",
day = "1",
doi = "10.1242/jcs.008417",
language = "English (US)",
volume = "120",
pages = "2652--2662",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "15",

}

TY - JOUR

T1 - Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling

AU - Bhattacharya, Sumitra

AU - Das, Ani

AU - Mallya, Kavita

AU - Ahmad, Iqbal

PY - 2007/8/1

Y1 - 2007/8/1

N2 - ABCG2 belongs to the ATP-binding cassette superfamily of transmembrane proteins and is ubiquitously expressed in stem cells including those in the developing nervous system. The ability of ABCG2 to preferentially exclude DNA-intercalating dyes is regarded to be the basis for the enrichment of stem cells or progenitors as dyelow side population (SP) cells. However, the role of ABCG2 in neural stem cells remains speculative and poorly understood. Here, we demonstrate using retinal stem cells, that ABCG2 is the molecular determinant of SP cell phenotype of neural stem cells and plays an important role in their maintenance. Overexpression of ABCG2 prevents the SP cell phenotype and adversely affects the lineage commitment of retinal stem cells. By contrast, targeted attenuation of ABCG2 depletes retinal SP cells and promotes their differentiation along pan neural and retinal lineages. In addition, we demonstrate for the first time that ABCG2 is a target of Notch signaling, and as such, constitutes one of the genes in the regulatory network of Notch signaling, involved in the maintenance of stem cells.

AB - ABCG2 belongs to the ATP-binding cassette superfamily of transmembrane proteins and is ubiquitously expressed in stem cells including those in the developing nervous system. The ability of ABCG2 to preferentially exclude DNA-intercalating dyes is regarded to be the basis for the enrichment of stem cells or progenitors as dyelow side population (SP) cells. However, the role of ABCG2 in neural stem cells remains speculative and poorly understood. Here, we demonstrate using retinal stem cells, that ABCG2 is the molecular determinant of SP cell phenotype of neural stem cells and plays an important role in their maintenance. Overexpression of ABCG2 prevents the SP cell phenotype and adversely affects the lineage commitment of retinal stem cells. By contrast, targeted attenuation of ABCG2 depletes retinal SP cells and promotes their differentiation along pan neural and retinal lineages. In addition, we demonstrate for the first time that ABCG2 is a target of Notch signaling, and as such, constitutes one of the genes in the regulatory network of Notch signaling, involved in the maintenance of stem cells.

KW - ABCG2

KW - Notch

KW - Progenitors

KW - Retina

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=34548253014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548253014&partnerID=8YFLogxK

U2 - 10.1242/jcs.008417

DO - 10.1242/jcs.008417

M3 - Article

VL - 120

SP - 2652

EP - 2662

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 15

ER -