Magnesium sulfate induces translocation of protein kinase C isoenzymes alpha and delta in myometrial cells from pregnant women

Miguel E. Bermeo, Victor P. Fomin, Gary Ventolini, Shawn G. Gibbs, David S. McKenna, William W. Hurd

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives: The purpose of this study was to determine the effect of magnesium sulfate on protein kinase C (PKC) translocation in myometrial cells from pregnant women. Study design: Myometrium was obtained at the time of cesarean delivery from women at term before labor. Cultured myometrial cells were treated with magnesium sulfate (3, 5. and 10 mmol/L). oxytocin (0.1 μmol/L), or 12-O-tetradecanoylphorbol-13-acetate (TPA, 0.1 μmol/L). The translocation of PKC isozymes alpha (calcium dependent) and delta (calcium independent) from cytosol to membrane fractions was assessed with use of Western blot analysis. Results: In unexposed control cells, the majority of PKC alpha and delta was located in the cytosol fraction. Exposure to magnesium sulfate for 60 minutes induced translocation of both PKC alpha and delta at concentrations as low as 5 and 3 mmol/L. respectively. The magnitude of magnesium sulfate induced translocation for PKC delta is similar to that seen after oxytocin or TPA exposure but less for PKC alpha. Exposure to oxytocin for 30 minutes and 60 minutes induced translocation of PKC alpha and delta, respectively. Exposure to TPA for 5 and 30 minutes induced translocation of PKC alpha and PKC delta, respectively. In calcium-free media, only TPA induced translocation of these two isoenzymes. Conclusion: Magnesium sulfate stimulates PKC translocation in cultured myometrial cells from pregnant women. Magnesium sulfate and oxytocin require extracellular calcium to induce translocation of both PKC alpha and delta.

Original languageEnglish (US)
Pages (from-to)522-527
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume190
Issue number2
DOIs
StatePublished - Feb 2004

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Protein Kinase C-delta
Protein Kinase C-alpha
Magnesium Sulfate
Isoenzymes
Pregnant Women
Oxytocin
Calcium
Cytosol
Protein Kinase C
Cultured Cells
Myometrium
Tetradecanoylphorbol Acetate
Western Blotting
Membranes

Keywords

  • Magnesium sulfate
  • Myometrium
  • Oxytocin
  • Pregnancy
  • Protein kinase C
  • Uterus

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Magnesium sulfate induces translocation of protein kinase C isoenzymes alpha and delta in myometrial cells from pregnant women. / Bermeo, Miguel E.; Fomin, Victor P.; Ventolini, Gary; Gibbs, Shawn G.; McKenna, David S.; Hurd, William W.

In: American Journal of Obstetrics and Gynecology, Vol. 190, No. 2, 02.2004, p. 522-527.

Research output: Contribution to journalArticle

Bermeo, Miguel E. ; Fomin, Victor P. ; Ventolini, Gary ; Gibbs, Shawn G. ; McKenna, David S. ; Hurd, William W. / Magnesium sulfate induces translocation of protein kinase C isoenzymes alpha and delta in myometrial cells from pregnant women. In: American Journal of Obstetrics and Gynecology. 2004 ; Vol. 190, No. 2. pp. 522-527.
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abstract = "Objectives: The purpose of this study was to determine the effect of magnesium sulfate on protein kinase C (PKC) translocation in myometrial cells from pregnant women. Study design: Myometrium was obtained at the time of cesarean delivery from women at term before labor. Cultured myometrial cells were treated with magnesium sulfate (3, 5. and 10 mmol/L). oxytocin (0.1 μmol/L), or 12-O-tetradecanoylphorbol-13-acetate (TPA, 0.1 μmol/L). The translocation of PKC isozymes alpha (calcium dependent) and delta (calcium independent) from cytosol to membrane fractions was assessed with use of Western blot analysis. Results: In unexposed control cells, the majority of PKC alpha and delta was located in the cytosol fraction. Exposure to magnesium sulfate for 60 minutes induced translocation of both PKC alpha and delta at concentrations as low as 5 and 3 mmol/L. respectively. The magnitude of magnesium sulfate induced translocation for PKC delta is similar to that seen after oxytocin or TPA exposure but less for PKC alpha. Exposure to oxytocin for 30 minutes and 60 minutes induced translocation of PKC alpha and delta, respectively. Exposure to TPA for 5 and 30 minutes induced translocation of PKC alpha and PKC delta, respectively. In calcium-free media, only TPA induced translocation of these two isoenzymes. Conclusion: Magnesium sulfate stimulates PKC translocation in cultured myometrial cells from pregnant women. Magnesium sulfate and oxytocin require extracellular calcium to induce translocation of both PKC alpha and delta.",
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AU - McKenna, David S.

AU - Hurd, William W.

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N2 - Objectives: The purpose of this study was to determine the effect of magnesium sulfate on protein kinase C (PKC) translocation in myometrial cells from pregnant women. Study design: Myometrium was obtained at the time of cesarean delivery from women at term before labor. Cultured myometrial cells were treated with magnesium sulfate (3, 5. and 10 mmol/L). oxytocin (0.1 μmol/L), or 12-O-tetradecanoylphorbol-13-acetate (TPA, 0.1 μmol/L). The translocation of PKC isozymes alpha (calcium dependent) and delta (calcium independent) from cytosol to membrane fractions was assessed with use of Western blot analysis. Results: In unexposed control cells, the majority of PKC alpha and delta was located in the cytosol fraction. Exposure to magnesium sulfate for 60 minutes induced translocation of both PKC alpha and delta at concentrations as low as 5 and 3 mmol/L. respectively. The magnitude of magnesium sulfate induced translocation for PKC delta is similar to that seen after oxytocin or TPA exposure but less for PKC alpha. Exposure to oxytocin for 30 minutes and 60 minutes induced translocation of PKC alpha and delta, respectively. Exposure to TPA for 5 and 30 minutes induced translocation of PKC alpha and PKC delta, respectively. In calcium-free media, only TPA induced translocation of these two isoenzymes. Conclusion: Magnesium sulfate stimulates PKC translocation in cultured myometrial cells from pregnant women. Magnesium sulfate and oxytocin require extracellular calcium to induce translocation of both PKC alpha and delta.

AB - Objectives: The purpose of this study was to determine the effect of magnesium sulfate on protein kinase C (PKC) translocation in myometrial cells from pregnant women. Study design: Myometrium was obtained at the time of cesarean delivery from women at term before labor. Cultured myometrial cells were treated with magnesium sulfate (3, 5. and 10 mmol/L). oxytocin (0.1 μmol/L), or 12-O-tetradecanoylphorbol-13-acetate (TPA, 0.1 μmol/L). The translocation of PKC isozymes alpha (calcium dependent) and delta (calcium independent) from cytosol to membrane fractions was assessed with use of Western blot analysis. Results: In unexposed control cells, the majority of PKC alpha and delta was located in the cytosol fraction. Exposure to magnesium sulfate for 60 minutes induced translocation of both PKC alpha and delta at concentrations as low as 5 and 3 mmol/L. respectively. The magnitude of magnesium sulfate induced translocation for PKC delta is similar to that seen after oxytocin or TPA exposure but less for PKC alpha. Exposure to oxytocin for 30 minutes and 60 minutes induced translocation of PKC alpha and delta, respectively. Exposure to TPA for 5 and 30 minutes induced translocation of PKC alpha and PKC delta, respectively. In calcium-free media, only TPA induced translocation of these two isoenzymes. Conclusion: Magnesium sulfate stimulates PKC translocation in cultured myometrial cells from pregnant women. Magnesium sulfate and oxytocin require extracellular calcium to induce translocation of both PKC alpha and delta.

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