Macrophage-induced inflammation affects hippocampal plasticity and neuronal development in a murine model of HIV-1 encephalitis

Larisa Y Poluektova, Vakara Meyer, Lisa Walters, Ximena Paez, Howard Eliot Gendelman

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Cognitive, behavioral, and motor impairments, during progressive human immunodeficiency virus type 1 (HIV-1) infection, are linked to activation of brain mononuclear phagocytes (MP; perivascular macrophages and microglia). Activated MPs effect a giant cell encephalitis and neuroinflammatory responses that are mirrored in severe combined immunodeficient (SCID) mice injected with human monocyte-derived macrophages (MDM). Whether activated human MDMs positioned in the basal ganglia affect hippocampal neuronal plasticity, the brain subregion involved in learning and memory, is unknown. Thus, immunohistochemical techniques were used for detection of newborn neurons (polysialylated neuronal cell adhesion molecule [PSA-NCAM]) and cell proliferation (Ki-67) to assay MDM effects on neuronal development in mouse models of HIV-1 encephalitis. Immunodeficient (C.B.-17/SCID and nonobese diabetic/SCID, NOD/SCID) and immune competent (C.B.-17) mice were injected with uninfected or HIV-1-infected MDM. Sham-operated or unmanipulated mice served as controls. Neuronal plasticity was evaluated in the hippocampal dentate gyrus (DG) at days 7 and 28. By day 7, increased numbers of Ki-67+ cells, PSA-NCAM+ cells and dendrites in DG were observed in sham-operated animals. In contrast, significant reductions in neuronal precursors and altered neuronal morphology paralleled increased microglial activation in both HIV-1-infected and uninfected MDM-injected animals. DG cellular composition was restored at day 28. We posit that activated MDM induce inflammation and diminish DG neuronal plasticity. These data provide novel explanations for the cognitive impairments manifested during advanced HIV-1 infection.

Original languageEnglish (US)
Pages (from-to)344-353
Number of pages10
JournalGlia
Volume52
Issue number4
DOIs
StatePublished - Dec 1 2005

Fingerprint

Neuronal Plasticity
Encephalitis
HIV-1
Macrophages
Dentate Gyrus
Inflammation
Neuronal Cell Adhesion Molecules
Virus Diseases
Parahippocampal Gyrus
SCID Mice
Brain
Microglia
Giant Cells
Phagocytes
Dendrites
Basal Ganglia
Cell Proliferation
Learning
Neurons

Keywords

  • Astrocytes
  • Cell proliferation
  • Hippocampus
  • Ki-67
  • Microglia
  • Neurons
  • PSA-NCAM

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Macrophage-induced inflammation affects hippocampal plasticity and neuronal development in a murine model of HIV-1 encephalitis. / Poluektova, Larisa Y; Meyer, Vakara; Walters, Lisa; Paez, Ximena; Gendelman, Howard Eliot.

In: Glia, Vol. 52, No. 4, 01.12.2005, p. 344-353.

Research output: Contribution to journalArticle

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