Macrophage bridging conduit trafficking of HIV-1 through the endoplasmic reticulum and golgi network

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Bridging conduits (BC) are tubular protrusions that facilitate cytoplasm and membrane exchanges between tethered cells. We now report that the human immunodeficiency virus type I (HIV-1) exploits these conduits to accelerate its spread and to shield it from immune surveillance. Endosome transport through BC drives HIV-1 intercellular transfers. How this occurs was studied in human monocyte-derived macrophages using proteomic, biochemical, and imaging techniques. Endosome, endoplasmic reticulum (ER), Golgi markers, and HIV-1 proteins were identified by proteomic assays in isolated conduits. Both the ER and Golgi showed elongated and tubular morphologies that extended into the conduits of polarized macrophages. Env and Gag antigen and fluorescent HIV-1 tracking demonstrated that these viral constituents were sequestered into endocytic and ER-Golgi organelles. Sequestered infectious viral components targeted the Golgi and ER by retrograde transport from early and Rab9 late endosomes. Disruption of the ER-Golgi network impaired HIV-1 trafficking in the conduit endosomes. This study provides, for the first time, mechanisms for how BC Golgi and ER direct cell-cell viral transfer.

Original languageEnglish (US)
Pages (from-to)3225-3238
Number of pages14
JournalJournal of proteome research
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2011

Fingerprint

Macrophages
Endoplasmic Reticulum
HIV-1
Endosomes
env Gene Products
gag Gene Products
Viruses
Assays
Membranes
Imaging techniques
Proteomics
Human Immunodeficiency Virus Proteins
Viral Structures
Proteins
Organelles
Cytoplasm
HIV

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Macrophage bridging conduit trafficking of HIV-1 through the endoplasmic reticulum and golgi network. / Kadiu, Irena; Gendelman, Howard Eliot.

In: Journal of proteome research, Vol. 10, No. 7, 01.07.2011, p. 3225-3238.

Research output: Contribution to journalArticle

@article{ad1e3a2cc2654c98a7c65dabd4359194,
title = "Macrophage bridging conduit trafficking of HIV-1 through the endoplasmic reticulum and golgi network",
abstract = "Bridging conduits (BC) are tubular protrusions that facilitate cytoplasm and membrane exchanges between tethered cells. We now report that the human immunodeficiency virus type I (HIV-1) exploits these conduits to accelerate its spread and to shield it from immune surveillance. Endosome transport through BC drives HIV-1 intercellular transfers. How this occurs was studied in human monocyte-derived macrophages using proteomic, biochemical, and imaging techniques. Endosome, endoplasmic reticulum (ER), Golgi markers, and HIV-1 proteins were identified by proteomic assays in isolated conduits. Both the ER and Golgi showed elongated and tubular morphologies that extended into the conduits of polarized macrophages. Env and Gag antigen and fluorescent HIV-1 tracking demonstrated that these viral constituents were sequestered into endocytic and ER-Golgi organelles. Sequestered infectious viral components targeted the Golgi and ER by retrograde transport from early and Rab9 late endosomes. Disruption of the ER-Golgi network impaired HIV-1 trafficking in the conduit endosomes. This study provides, for the first time, mechanisms for how BC Golgi and ER direct cell-cell viral transfer.",
author = "Irena Kadiu and Gendelman, {Howard Eliot}",
year = "2011",
month = "7",
day = "1",
doi = "10.1021/pr200262q",
language = "English (US)",
volume = "10",
pages = "3225--3238",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Macrophage bridging conduit trafficking of HIV-1 through the endoplasmic reticulum and golgi network

AU - Kadiu, Irena

AU - Gendelman, Howard Eliot

PY - 2011/7/1

Y1 - 2011/7/1

N2 - Bridging conduits (BC) are tubular protrusions that facilitate cytoplasm and membrane exchanges between tethered cells. We now report that the human immunodeficiency virus type I (HIV-1) exploits these conduits to accelerate its spread and to shield it from immune surveillance. Endosome transport through BC drives HIV-1 intercellular transfers. How this occurs was studied in human monocyte-derived macrophages using proteomic, biochemical, and imaging techniques. Endosome, endoplasmic reticulum (ER), Golgi markers, and HIV-1 proteins were identified by proteomic assays in isolated conduits. Both the ER and Golgi showed elongated and tubular morphologies that extended into the conduits of polarized macrophages. Env and Gag antigen and fluorescent HIV-1 tracking demonstrated that these viral constituents were sequestered into endocytic and ER-Golgi organelles. Sequestered infectious viral components targeted the Golgi and ER by retrograde transport from early and Rab9 late endosomes. Disruption of the ER-Golgi network impaired HIV-1 trafficking in the conduit endosomes. This study provides, for the first time, mechanisms for how BC Golgi and ER direct cell-cell viral transfer.

AB - Bridging conduits (BC) are tubular protrusions that facilitate cytoplasm and membrane exchanges between tethered cells. We now report that the human immunodeficiency virus type I (HIV-1) exploits these conduits to accelerate its spread and to shield it from immune surveillance. Endosome transport through BC drives HIV-1 intercellular transfers. How this occurs was studied in human monocyte-derived macrophages using proteomic, biochemical, and imaging techniques. Endosome, endoplasmic reticulum (ER), Golgi markers, and HIV-1 proteins were identified by proteomic assays in isolated conduits. Both the ER and Golgi showed elongated and tubular morphologies that extended into the conduits of polarized macrophages. Env and Gag antigen and fluorescent HIV-1 tracking demonstrated that these viral constituents were sequestered into endocytic and ER-Golgi organelles. Sequestered infectious viral components targeted the Golgi and ER by retrograde transport from early and Rab9 late endosomes. Disruption of the ER-Golgi network impaired HIV-1 trafficking in the conduit endosomes. This study provides, for the first time, mechanisms for how BC Golgi and ER direct cell-cell viral transfer.

UR - http://www.scopus.com/inward/record.url?scp=79960025886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960025886&partnerID=8YFLogxK

U2 - 10.1021/pr200262q

DO - 10.1021/pr200262q

M3 - Article

C2 - 21563830

AN - SCOPUS:79960025886

VL - 10

SP - 3225

EP - 3238

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 7

ER -