MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling

Minhan Ka, Eui Man Jung, Ulrich Mueller, Woo Yang Kim

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Neuronal migration and subsequent differentiation play critical roles for establishing functional neural circuitry in the developing brain. However, the molecular mechanisms that regulate these processes are poorly understood. Here, we show that microtubule actin crosslinking factor 1 (MACF1) determines neuronal positioning by regulating microtubule dynamics and mediating GSK-3 signaling during brain development. First, using MACF1 floxed allele mice and in utero gene manipulation, we find that MACF1 deletion suppresses migration of cortical pyramidal neurons and results in aberrant neuronal positioning in the developing brain. The cell autonomous deficit in migration is associated with abnormal dynamics of leading processes and centrosomes. Furthermore, microtubule stability is severely damaged in neurons lacking MACF1, resulting in abnormal microtubule dynamics. Finally, MACF1 interacts with and mediates GSK-3 signaling in developing neurons. Our findings establish a cellular mechanism underlying neuronal migration and provide insights into the regulation of cytoskeleton dynamics in developing neurons.

Original languageEnglish (US)
Pages (from-to)4-18
Number of pages15
JournalDevelopmental Biology
Volume395
Issue number1
DOIs
StatePublished - Nov 1 2014

Fingerprint

Glycogen Synthase Kinase 3
Pyramidal Cells
Microtubules
Neurons
Brain
Centrosome
Cytoskeleton
Alleles
human MACF1 protein
Genes

Keywords

  • Cytoskeleton
  • GSK-3
  • MACF1
  • Microtubule
  • Neuronal migration

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. / Ka, Minhan; Jung, Eui Man; Mueller, Ulrich; Kim, Woo Yang.

In: Developmental Biology, Vol. 395, No. 1, 01.11.2014, p. 4-18.

Research output: Contribution to journalArticle

Ka, Minhan ; Jung, Eui Man ; Mueller, Ulrich ; Kim, Woo Yang. / MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. In: Developmental Biology. 2014 ; Vol. 395, No. 1. pp. 4-18.
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