MAC3A and MAC3B, two core subunits of the MOS4-associated complex, positively influence miRNA biogenesis

Shengjun Li, Kan Liu, Bangjun Zhou, Mu Li, Shuxin Zhang, Lirong Zeng, Chi Zhang, Bin Yu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

MAC3A and MAC3B are conserved U-box-containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis thaliana. However, their functional mechanisms remain elusive. Here, we show that Arabidopsis MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs (MIR genes), suggesting that they may not affect MIR transcription. This result, together with the fact that MAC3A associates with pri-miRNAs in vivo, indicates that MAC3A and MAC3B may stabilize pri-miRNAs. Furthermore, we find that MAC3A and MAC3B interact with the DCL1 complex that catalyzes miRNA maturation, promote DCL1 activity, and are required for the localization of HYL1, a component of the DCL1 complex. Besides MAC3A and MAC3B, two other MAC subunits, CDC5 and PRL1, also function in miRNA biogenesis. Based on these results, we propose that MAC functions as a complex to control miRNA levels through modulating pri-miRNA transcription, processing, and stability.

Original languageEnglish (US)
Pages (from-to)481-494
Number of pages14
JournalPlant Cell
Volume30
Issue number2
DOIs
StatePublished - Feb 2018

Fingerprint

MicroRNAs
microRNA
Arabidopsis
transcription (genetics)
biogenesis
Plant Immunity
Plant Development
eukaryotic cells
Eukaryota
genes
Arabidopsis thaliana
immunity
Genes
promoter regions
mutants

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

MAC3A and MAC3B, two core subunits of the MOS4-associated complex, positively influence miRNA biogenesis. / Li, Shengjun; Liu, Kan; Zhou, Bangjun; Li, Mu; Zhang, Shuxin; Zeng, Lirong; Zhang, Chi; Yu, Bin.

In: Plant Cell, Vol. 30, No. 2, 02.2018, p. 481-494.

Research output: Contribution to journalArticle

Li, Shengjun ; Liu, Kan ; Zhou, Bangjun ; Li, Mu ; Zhang, Shuxin ; Zeng, Lirong ; Zhang, Chi ; Yu, Bin. / MAC3A and MAC3B, two core subunits of the MOS4-associated complex, positively influence miRNA biogenesis. In: Plant Cell. 2018 ; Vol. 30, No. 2. pp. 481-494.
@article{dfb23b1a4801407ebc595b69a26bc4ad,
title = "MAC3A and MAC3B, two core subunits of the MOS4-associated complex, positively influence miRNA biogenesis",
abstract = "MAC3A and MAC3B are conserved U-box-containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis thaliana. However, their functional mechanisms remain elusive. Here, we show that Arabidopsis MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs (MIR genes), suggesting that they may not affect MIR transcription. This result, together with the fact that MAC3A associates with pri-miRNAs in vivo, indicates that MAC3A and MAC3B may stabilize pri-miRNAs. Furthermore, we find that MAC3A and MAC3B interact with the DCL1 complex that catalyzes miRNA maturation, promote DCL1 activity, and are required for the localization of HYL1, a component of the DCL1 complex. Besides MAC3A and MAC3B, two other MAC subunits, CDC5 and PRL1, also function in miRNA biogenesis. Based on these results, we propose that MAC functions as a complex to control miRNA levels through modulating pri-miRNA transcription, processing, and stability.",
author = "Shengjun Li and Kan Liu and Bangjun Zhou and Mu Li and Shuxin Zhang and Lirong Zeng and Chi Zhang and Bin Yu",
year = "2018",
month = "2",
doi = "10.1105/tpc.17.00953",
language = "English (US)",
volume = "30",
pages = "481--494",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "2",

}

TY - JOUR

T1 - MAC3A and MAC3B, two core subunits of the MOS4-associated complex, positively influence miRNA biogenesis

AU - Li, Shengjun

AU - Liu, Kan

AU - Zhou, Bangjun

AU - Li, Mu

AU - Zhang, Shuxin

AU - Zeng, Lirong

AU - Zhang, Chi

AU - Yu, Bin

PY - 2018/2

Y1 - 2018/2

N2 - MAC3A and MAC3B are conserved U-box-containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis thaliana. However, their functional mechanisms remain elusive. Here, we show that Arabidopsis MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs (MIR genes), suggesting that they may not affect MIR transcription. This result, together with the fact that MAC3A associates with pri-miRNAs in vivo, indicates that MAC3A and MAC3B may stabilize pri-miRNAs. Furthermore, we find that MAC3A and MAC3B interact with the DCL1 complex that catalyzes miRNA maturation, promote DCL1 activity, and are required for the localization of HYL1, a component of the DCL1 complex. Besides MAC3A and MAC3B, two other MAC subunits, CDC5 and PRL1, also function in miRNA biogenesis. Based on these results, we propose that MAC functions as a complex to control miRNA levels through modulating pri-miRNA transcription, processing, and stability.

AB - MAC3A and MAC3B are conserved U-box-containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis thaliana. However, their functional mechanisms remain elusive. Here, we show that Arabidopsis MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs (MIR genes), suggesting that they may not affect MIR transcription. This result, together with the fact that MAC3A associates with pri-miRNAs in vivo, indicates that MAC3A and MAC3B may stabilize pri-miRNAs. Furthermore, we find that MAC3A and MAC3B interact with the DCL1 complex that catalyzes miRNA maturation, promote DCL1 activity, and are required for the localization of HYL1, a component of the DCL1 complex. Besides MAC3A and MAC3B, two other MAC subunits, CDC5 and PRL1, also function in miRNA biogenesis. Based on these results, we propose that MAC functions as a complex to control miRNA levels through modulating pri-miRNA transcription, processing, and stability.

UR - http://www.scopus.com/inward/record.url?scp=85043756611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043756611&partnerID=8YFLogxK

U2 - 10.1105/tpc.17.00953

DO - 10.1105/tpc.17.00953

M3 - Article

C2 - 29437988

AN - SCOPUS:85043756611

VL - 30

SP - 481

EP - 494

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 2

ER -