M2 proton channel structural validation from full-length protein samples in synthetic bilayers and E. coli membranes

Yimin Miao, Huajun Qin, Riqiang Fu, Mukesh Sharma, Thach V. Can, Ivan Hung, Sorin Luca, Peter L. Gor'Kov, William W. Brey, Timothy A. Cross

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Validation: Membrane protein structures are sensitive to the environment used for structural characterization. NMR spectra of the full-length M2 proton channel from influenza A were measured directly in E. coli membranes and compared to spectra of the protein in synthetic lipid bilayers. The results demonstrate that these bilayers provide a native-like membrane environment.

Original languageEnglish (US)
Pages (from-to)8383-8386
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number33
DOIs
StatePublished - Aug 13 2012

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Escherichia coli
Protons
Proteins
Membranes
Lipid bilayers
Membrane Proteins
Nuclear magnetic resonance

Keywords

  • M2 protein
  • NMR spectroscopy
  • membrane proteins
  • structural biology
  • structural validation

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

M2 proton channel structural validation from full-length protein samples in synthetic bilayers and E. coli membranes. / Miao, Yimin; Qin, Huajun; Fu, Riqiang; Sharma, Mukesh; Can, Thach V.; Hung, Ivan; Luca, Sorin; Gor'Kov, Peter L.; Brey, William W.; Cross, Timothy A.

In: Angewandte Chemie - International Edition, Vol. 51, No. 33, 13.08.2012, p. 8383-8386.

Research output: Contribution to journalArticle

Miao, Y, Qin, H, Fu, R, Sharma, M, Can, TV, Hung, I, Luca, S, Gor'Kov, PL, Brey, WW & Cross, TA 2012, 'M2 proton channel structural validation from full-length protein samples in synthetic bilayers and E. coli membranes', Angewandte Chemie - International Edition, vol. 51, no. 33, pp. 8383-8386. https://doi.org/10.1002/anie.201204666
Miao, Yimin ; Qin, Huajun ; Fu, Riqiang ; Sharma, Mukesh ; Can, Thach V. ; Hung, Ivan ; Luca, Sorin ; Gor'Kov, Peter L. ; Brey, William W. ; Cross, Timothy A. / M2 proton channel structural validation from full-length protein samples in synthetic bilayers and E. coli membranes. In: Angewandte Chemie - International Edition. 2012 ; Vol. 51, No. 33. pp. 8383-8386.
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