Lysophosphatidic acid enhances contractility of isolated airway smooth muscle

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The effects of the simple phospholipid mediator lysophosphatidic acid (LPA) on the contractile responsiveness of isolated tracheal rings from rabbits and cats were assessed. In both species, LPA increased the contractile response to the muscarinic agonist methacholine, but LPA did not induce contraction on its own. Conversely, LPA decreased the relaxation response to the β-adrenergic-agonist isoproterenol in both species. Concentrations of LPA as low as 10-8 M were effective, and the effects of LPA were rapidly reversed on washrag. Phosphatidic acid was much less effective, requiring higher concentrations and producing only a minimal effect. Contractions induced by serotonin and by substance P also were enhanced by LPA, but KCl-induced contractions were unaffected. LPA inhibited the isoproterenol-induced relaxation of KCl-precontracted rings, similar to its effects on methacholine-precontracted rings, and relaxation induced by the direct adenylyl cyclase activator forskolin was inhibited in a manner similar to that induced by isoproterenol. Epithelium removal did not alter the contraction-enhancing effect of LPA. The ability of LPA to both enhance contraction and inhibit relaxation of airway smooth muscle suggests that LPA could contribute to airway hypercontractility in asthma, airway inflammation, or other types of lung injury.

Original languageEnglish (US)
Pages (from-to)1216-1222
Number of pages7
JournalJournal of Applied Physiology
Volume83
Issue number4
StatePublished - Oct 1 1997

Fingerprint

Smooth Muscle
Isoproterenol
Methacholine Chloride
lysophosphatidic acid
Muscarinic Agonists
Adrenergic Agonists
Phosphatidic Acids
Lung Injury
Colforsin
Substance P
Adenylyl Cyclases
Phospholipids
Serotonin
Cats
Asthma
Epithelium
Rabbits
Inflammation

Keywords

  • Bronchoconstriction

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Lysophosphatidic acid enhances contractility of isolated airway smooth muscle. / Toews, Myron Lee; Ustinova, E. E.; Schultz, Harold D.

In: Journal of Applied Physiology, Vol. 83, No. 4, 01.10.1997, p. 1216-1222.

Research output: Contribution to journalArticle

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