Lysobacter enzymogenes uses two distinct cell-cell signaling systems for differential regulation of secondary-metabolite biosynthesis and colony morphology

Guoliang Qian, Yulan Wang, Yiru Liu, Feifei Xu, Ya Wen He, Liangcheng Du, Vittorio Venturi, Jiaqin Fan, Baishi Hu, Fengquan Liu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Lysobacter enzymogenes is a ubiquitous environmental bacterium that is emerging as a potentially novel biological control agent and a new source of bioactive secondary metabolites, such as the heat-stable antifungal factor (HSAF) and photoprotective polyene pigments. Thus far, the regulatory mechanism(s) for biosynthesis of these bioactive secondary metabolites remains largely unknown in L. enzymogenes. In the present study, the diffusible signal factor (DSF) and diffusible factor (DF)-mediated cell-cell signaling systems were identified for the first time from L. enzymogenes. The results show that both Rpf/DSF and DF signaling systems played critical roles in modulating HSAF biosynthesis in L. enzymogenes. Rpf/DSF signaling and DF signaling played negative and positive effects in polyene pigment production, respectively, with DF playing a more important role in regulating this phenotype. Interestingly, only Rpf/DSF, but not the DF signaling system, regulated colony morphology of L. enzymgenes. Both Rpf/DSF and DF signaling systems were involved in the modulation of expression of genes with diverse functions in L. enzymogenes, and their own regulons exhibited only a few loci that were regulated by both systems. These findings unveil for the first time new roles of the Rpf/DSF and DF signaling systems in secondary metabolite biosynthesis of L. enzymogenes.

Original languageEnglish (US)
Pages (from-to)6604-6616
Number of pages13
JournalApplied and environmental microbiology
Volume79
Issue number21
DOIs
StatePublished - Oct 25 2013

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Lysobacter
Lysobacter enzymogenes
Polyenes
secondary metabolite
secondary metabolites
Hot Temperature
biosynthesis
Biological Control Agents
Regulon
cells
Bacteria
Phenotype
Gene Expression
pigment
pigments
heat
regulon
biological control agents
regulation
biological control

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Lysobacter enzymogenes uses two distinct cell-cell signaling systems for differential regulation of secondary-metabolite biosynthesis and colony morphology. / Qian, Guoliang; Wang, Yulan; Liu, Yiru; Xu, Feifei; He, Ya Wen; Du, Liangcheng; Venturi, Vittorio; Fan, Jiaqin; Hu, Baishi; Liu, Fengquan.

In: Applied and environmental microbiology, Vol. 79, No. 21, 25.10.2013, p. 6604-6616.

Research output: Contribution to journalArticle

Qian, Guoliang ; Wang, Yulan ; Liu, Yiru ; Xu, Feifei ; He, Ya Wen ; Du, Liangcheng ; Venturi, Vittorio ; Fan, Jiaqin ; Hu, Baishi ; Liu, Fengquan. / Lysobacter enzymogenes uses two distinct cell-cell signaling systems for differential regulation of secondary-metabolite biosynthesis and colony morphology. In: Applied and environmental microbiology. 2013 ; Vol. 79, No. 21. pp. 6604-6616.
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